食品工业科技2018,Vol.39Issue(2):17-21,5.DOI:10.13386/j.issn1002-0306.2018.02.004
番茄果胶和半纤维素抗氧化活性分析
Analysis of antioxidant activity of pectin and hemicellulose extracted from tomato fruit
摘要
Abstract
Water-soluble pectin (WSP),chelate-soluble pectin (CSP),sodium carbonate-soluble pectin (SSP),loosebonding hemicellulose(LH)and tight-bonding hemicellulose(TH)were extracted from tomato fruit cell wall with water,EDTA,Na2CO3,4% and 24% KOH.The antioxidant activity of different pectins and hemicellulose were studied,including oxygen radical absorbance capacity (ORAC),DPPH radical (DPPH ·) scavenging capacity,ABTS radical (ABTS + ·) scavenging capacity and ferric reducing antioxidant power(FRAP).It was suggested that the content of WSP(1.05 mg GalUA/g FW)was significantly higher than CSP and SSP in tomato fruit,and the content of TH (0.09 mg Glu/g FW)was significantly higher than LH(p < 0.05).And different pectins and hemicellulose from tomato fruit all showed antioxidant activity in different degree,CSP' s total antioxidant activity was higher than other cell wall components with higher ORAC,DPPH· scavenging capacity and ABTS + · scavenging capacity,when the concentration was 5 mg/mL,its ORAC value was 76.4 μmol/L Trolox equivalent,and the DPPH· scavenging rate and ABTS + · scavenging rate was 61.0% and 99.8%,respectively.But SSP,LH and TH possessed higher FRAP.Correlation analysis suggested each cell wall component was closely related with antioxidant activity.This result made a reference to comprehensive utilization of tomato fruit and the application of functional additive in food.关键词
番茄/细胞壁组分/果胶/半纤维素/抗氧化活性Key words
tomato/cell wall components/pectin/hemicellulose/antioxidant activity分类
轻工纺织引用本文复制引用
李倩倩,李武笋,桑伟娜,王丽敏,赵玉梅,曹建康..番茄果胶和半纤维素抗氧化活性分析[J].食品工业科技,2018,39(2):17-21,5.基金项目
国家重点基础研究发展计划(973计划)项目(2013CB127104) (973计划)
国家自然科学基金面上项目(31371846). (31371846)