食品工业科技2018，Vol.39Issue(2)：65-69,5.DOI:10.13386/j.issn1002-0306.2018.02.013
核桃仁皮不同极性提取物的体外抗氧化活性分析
Study on antioxidant activity of different polar extracts from walnut kernel pellicle in vitro
摘要
Abstract
Using ethyl acetate,n-butanol,chloroform,petroleum ether extracted polyphenols from walnut kernel pellicle.Preliminary evaluation the antioxidant activities of different polarity extract from walnut kernel pellicle by DPPH free radical scavening assay,OH free radical scavenging assay,ABTS free radical scavening assay and iron ion reduction capacity.The results showed that the extraction capacity of polyphenols from walnut kernel pellicle was ethyl acetate ＞ n-butanol ＞ chloroform ＞ petroleum ether.The ethyl acetate extract has the strongest DPPH free radical and ABTS free radical scavenging ability,the IC50 values were 5.004 μg/mL and 29.654 μg/mL,followed by the n-butanol (9.596,23.681 μg/mL),chloroform extract (62.719,144.475 μg/mL) and petroleum ether extract (48.316,146.424 μg/mL).The n-butanol extract has the strongest OH free radical scavenging capacity,the IC50 value was 393.578 μg/mL,followed by ethyl acetate(510.186 μg/mL),chloroform extract(627.003 μg/mL)and petroleum ether extract(840.315 μg/mL).When the sample concentration was 100 μg/mL,the absorbance of the different polarity extracts were ethyl acetate extracts (0.821) ＞ n-butanol extracts (0.509) ＞ chloroform extracts(0.406) ＞ petroleum ether extracts (0.142)in the iron ion reduction reaction.In conclusion,the antioxidant activity of different polar extracts was different,followed by acetic ether ＞ n-butanol ＞ chloroform ＞ petroleum ether,and positively correlated with the mass concentration.关键词
核桃仁皮/多酚/极性提取物/抗氧化Key words
walnut kernel pellicle/polyphenolics/polar extracts/antioxidant activity分类
轻工纺织引用本文复制引用
蒲成伟,阚欢,刘云..核桃仁皮不同极性提取物的体外抗氧化活性分析[J].食品工业科技,2018,39(2):65-69,5.基金项目
云南省林业厅应用基础研究(215644). （215644）
