食品工业科技2018，Vol.39Issue(2)：81-85,5.DOI:10.13386/j.issn1002-0306.2018.02.016
pH控制策略及pH反馈流加培养法提高罗氏产碱杆菌的聚羟基丁酸产量
pH control strategy and pH feedback flow culture method used to improve the production of polyhydroxybutyrate produced by Roche alcaligenes sp
摘要
Abstract
In this passage,the biomass and production of polyhydroxybutyrate (PHB)were studied by controlling different pH values and using pH feedback to control the flow adding mode of medium.According to the experiments of batch culture and different culture medium,the pH feedback medium was confirmed.With this method,400 g/L glucose and 100 g/L yeast dip powder were added to the fermentation process,and the pH of the process was controlled at 6.7～6.9.Under these conditions,the dry weight of Roche alcaligenes sp was 22.52 g/L,and the yield of PHB reached 13.61 g/L.The biomass and PHB yield of Bacillus alcaligenes increased by 12.44％ and 5.07％.The results show that PHB can be improved by the pH feedback control medium.关键词
罗氏产碱杆菌/聚羟基丁酸/pHKey words
Roche alcaligenes sp/polyhydroxybutyrate/pH分类
轻工纺织引用本文复制引用
邓志伟,王莹莹,张永杰,王松廷,杨生玉..pH控制策略及pH反馈流加培养法提高罗氏产碱杆菌的聚羟基丁酸产量[J].食品工业科技,2018,39(2):81-85,5.基金项目
河南省科技攻关重点项目(152102210254). （152102210254）
