食品工业科技2018，Vol.39Issue(2)：92-97,6.DOI:10.13386/j.issn1002-0306.2018.02.018
蚕蛹蛋白的酶法水解工艺及其口服液的制备
Process of enzymatic hydrolysis of silkworm pupa and preparation of its oral liquid
摘要
Abstract
In order to obtain oral liquid of immunoreactive peptide from silkworm pupa protein,the enzymatic hydrolysis of silkworm pupa protein was optimized.In this paper,the enzymatic hydrolysis of skimmed silkworm pupa protein was carried out by using five enzymes,such as bromelain,alkaline protease,neutral protease,flavor protease and papain.The degree of hydrolysis and the proliferation of mouse spleen cells were used as indicators to obtain alkaline protease.The optimum enzymatic conditions were as follows:the enzymolysis temperature was 55 ℃,the amount of enzyme was 6％,the enzymolysis time was 2 h,pH8 was determined by single factor and response surface experiment,water and substrate ratio 20∶ 1,under this condition the degree of hydrolysis was 19.96％ ± 1.02％,immunoactive OD490 was 0.2512 ± 0.0125.And the preparation of silkworm pupa protein peptide immunoreactive oral liquid was studied.The optimum process was obtained by sensory evaluation that the amount of sugar was 8％ and citric acid was 1％.关键词
蚕蛹蛋白/酶解/口服液/响应面法Key words
silkworm pupa protein/enzymatic hydrolysis/oral liquid/response surface methodology分类
轻工纺织引用本文复制引用
黄美佳,戴燕,程剑锋,徐玉娟,邹宇晓,李欣..蚕蛹蛋白的酶法水解工艺及其口服液的制备[J].食品工业科技,2018,39(2):92-97,6.基金项目
2013年广东省教育部产学研专项资金(2013B090600060) （2013B090600060）
国家高技术研究发展计划(863计划)(2013AA102205) （863计划）
国家自然科学基金青年科学基金项目(31260204,31301522). （31260204,31301522）
