食品工业科技2018，Vol.39Issue(2)：102-107,6.DOI:10.13386/j.issn1002-0306.2018.02.020
晚熟桃采后褐腐病致病菌的分离鉴定及拮抗菌对其防治效果的研究
Isolation and identification of pathogen caused postharvest brown rot of late-ripening peach and bio-control effects of antagonistic bacteria
摘要
Abstract
In order to define the main pathogen caused postharvest brown rot of late-ripening peach and control effect of antagonistic bacteria,"Yingshuanghong" late-ripening peaches were used as the test materials.Then traditional morphological methods and 18S-rDNA molecular biology method was used to identify the pathogen caused postharvest brown rot of lateripening peach.At the same time,four kinds of antagonistic bacteria were used to prevent postharvest brown rot of late-ripening peach.Results showed that,in this study,pathogen separated from brown rot of late-ripening peach belongs to fungi,Ascomycota,Ascomycetes,Helotiales,Sclerotiniaceae,Sclerotinia and the fungi injury incidence was 100％.The inhibition effect of four kinds of Bacillus B001,B1,HB-2,and B579 to brown rot disease was significant.The inhibitory effect of B001 and B579 ahead of 1 d or 2 d prevention treatment was inhibitory effect significantly higher than 0 d treatment (p ＜ 0.05).The inhibitory effect of B1 and HB-2 ahead of 1 d prevention treatment was significantly higher than the other two groups (p ＜ 0.05).At the same time,the four kinds of antagonistic bacteria,a certain extent,could inhibit the decrease of VC content,soluble solids content,titratable acid content and hardness of late-ripening peach.The antagonistic bacteria,which in biological control of postharvest brown rot of late-ripening peach,has good application potential.关键词
晚熟桃/褐腐病/分离鉴定/品质分析/18S-rDNAKey words
late-ripening peach/brown rot/separated and identified/quality analysis/18S-rDNA分类
轻工纺织引用本文复制引用
张娜,郑香香,于晋泽,阎瑞香,关文强..晚熟桃采后褐腐病致病菌的分离鉴定及拮抗菌对其防治效果的研究[J].食品工业科技,2018,39(2):102-107,6.基金项目
国家科技支撑项目(2015BAD16B02). （2015BAD16B02）
