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基于单分子实时测序技术的3个当阳鲊广椒样品细菌多样性研究

王玉荣 孙永坤 代凯文 孙京新 沈馨 郭壮

食品工业科技2018,Vol.39Issue(2):108-112,118,6.
食品工业科技2018,Vol.39Issue(2):108-112,118,6.DOI:10.13386/j.issn1002-0306.2018.02.021

基于单分子实时测序技术的3个当阳鲊广椒样品细菌多样性研究

Study on the diversity of bacteria microflora in 3 Zhaguangjiao samples of Dangyang area by single molecule real-time sequencing technique

王玉荣 1孙永坤 2代凯文 2孙京新 3沈馨 1郭壮1

作者信息

  • 1. 湖北文理学院化学工程与食品科学学院,鄂西北传统发酵食品研究所,湖北襄阳441053
  • 2. 当阳市食品药品监督管理局,湖北宜昌444100
  • 3. 青岛农业大学食品科学与工程学院,山东青岛266109
  • 折叠

摘要

Abstract

In the current study,3 bacterial microbiota samples of Zhaguangjiao collected from Dangyang Hubei province were studied by single molecule real-time sequencing technique.Lactobacillus was the dominant bacteria genus with the relatively abundant of 94.25%.At the species level,relatively abundant species more than 1.0% of total bacteria in this population included L.pobuzih ii,L.alimentarius,L.versmoldensis,L.parabrevis,L.brevis,L.koreensis,and L.par alimentarius of Lactobacillus,with the relative abundance of 32.31%,23.17%,22.01%,8.90%,3.31%,1.63% and 1.05%.And W.paramesenteroides of Weissella had 1.05% of total bacteria.At the OTU level,the result indicated that 6OTUs shared by all samples,and the cumulative average relative was 29.58% of tutal qualified sequences.The results also showed that the bacterial microbiota of Zhaguangjiao samples main consisted by several bacterial species belonged to Lactobacillus,and the three samples shared a large number of core bacterial microbiome.

关键词

鲊广椒/单分子实时测序技术/细菌/多样性

Key words

Zhaguangjiao/single molecule real-time sequencing technique/bacterium/diversity

分类

轻工纺织

引用本文复制引用

王玉荣,孙永坤,代凯文,孙京新,沈馨,郭壮..基于单分子实时测序技术的3个当阳鲊广椒样品细菌多样性研究[J].食品工业科技,2018,39(2):108-112,118,6.

基金项目

湖北省高等学校优秀中青年科技创新团队计划项目(T201616) (T201616)

湖北文理学院教师科研能力培育基金(2017KYPY051). (2017KYPY051)

食品工业科技

OA北大核心CSTPCD

1002-0306

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