食品工业科技2018，Vol.39Issue(2)：128-133,6.DOI:10.13386/j.issn1002-0306.2018.02.025
酶解辅助乙醇提取牡丹果皮木犀草素的研究
Enzyme assisted ethanol extraction of luteolin from Paeonias suffruticosa pericarp
摘要
Abstract
Using the method of enzyme assisted ethanol extraction of luteolin in Paeonias suffruticosa "Feng Dan" pericarp.Firstly,taking the yield of luteolin as assessment index,effects of volume fraction of ethanol,soak time,liquid to solid ratio,the extraction times were investigated,and the optimal combination was discovered by orthogonal experiment on the basis of single factor experiment.On this basis,effects of pectinase amount,water bath time,pH,water bath temperature were investigated,and the optimal combination was discovered by orthogonal experiment on the basis of single factor experiment.The optimal factors conbination was as follows:the pericarp pretreated with pectinase content of 0.05 g·L-1,water bath time of 160 minutes,pH4.0,water bath temperature of 55 ℃,and then extracted in 60％ ethanol for 30 min,liquid to solid ratio of 10∶ 1,extract for 3 times,and the maximum yield of luteolin was up to 19.30 mg·g-1.关键词
“凤丹”牡丹果皮/木犀草素/果胶酶/提取Key words
Paeonias suffruticosa "Feng Dan" pericarp/luteolin/pectinase/extraction分类
轻工纺织引用本文复制引用
陈刚,杨玉珍,张敏,罗青,王国霞..酶解辅助乙醇提取牡丹果皮木犀草素的研究[J].食品工业科技,2018,39(2):128-133,6.基金项目
河南省科技攻关项目(172102110219) （172102110219）
郑州市科技计划项目(153PKJGG148) （153PKJGG148）
河南省高等学校重点科研项目(16B550008) （16B550008）
郑州师范学院科技创新团队支持计划. （）
