食品工业科技2018，Vol.39Issue(2)：207-211,216,6.DOI:10.13386/j.issn1002-0306.2018.02.039
铁皮石斛快繁体系多糖积累的研究
Study on the polysaccharide accumulation of Dendrobium officinale in rapid propagation
娄文娟 1欧阳凡 2董文宾2
作者信息
- 1. 河南科技学院食品学院,河南新乡453003
- 2. 陕西科技大学食品与生物工程学院,陕西西安710021
- 折叠
摘要
Abstract
To obtain fast-breeding and high medicinal-value.D.officinale,polysaccharide content was identifying indicator.Taking PLB as a carrier,Nitrate/ammonium molecule ratio,sucrose and BA concentration were recognized the most important factors in seven medium compositions affecting PLBs polysaccharide content through the Plackett-Burman design.The steepest ascent experiment and further Box-Behnken design response surface methods were designed,optimal results of the presence of regression model were as follows:nitrate/ammonium molecule ratio,sucrose and BA concentration were 1.90∶1,29.1 g/L,1.65 mg/L.Under these conditions,the mass fraction of the polysaccharide increased 33.95％ with a significant improvement compared with the value of 25.36％ before optimization.The optimization effect was remarkable.关键词
铁皮石斛/快繁体系/多糖Key words
Dendrobium officinale/rapid propagation/polysaccharide分类
轻工纺织引用本文复制引用
娄文娟,欧阳凡,董文宾..铁皮石斛快繁体系多糖积累的研究[J].食品工业科技,2018,39(2):207-211,216,6.
