食品科学2018，Vol.39Issue(1)：36-41,6.DOI:10.7506/spkx1002-6630-201801005
尖吻鲈鱼鳞和鱼皮胶原蛋白的提取及其理化特性分析
Extraction and Characterization of Collagen from Scales and Skin of Asian Seabass
摘要
Abstract
Pepsin-soluble collagen was extracted from the scales and skin of Asian seabass,and their physicochemical properties were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE),amino acid composition analysis,differential scanning calorimetry (DSC),Fourier transform infrared (FT-IR) spectroscopy,X-ray diffraction,zeta potential and solubility measurement.The yields of scale collagen and skin collagen were 2.3 and 47.3 g/100 g (dry weight),respectively.The SDS-PAGE profile showed that both collagens contained [α1(Ⅰ)]2α2(Ⅰ) and were characterized as type I collagen.DSC indicated that denaturation temperatures (Td) of scale and skin collagen were 37.54 and 36.74 ℃,respectively.Based on Fr-IR spectra and X-ray diffraction spectra,after pepsin treatment,the triple-helical structure of the collagens was still intact.Zeta potential studies indicated that scale and skin collagen had a net charge of zero at pH 6.40 and 6.64,respectively.Both collagens exhibited high solubility under acidic and low salt concentration conditions.关键词
尖吻鲈/鱼鳞/鱼皮/胶原蛋白/氨基酸Key words
Asian seabass/scale/skin/collagen/amino acid分类
轻工纺织引用本文复制引用
廖伟,夏光华,李川,仇昶旭,李永成,申铉日..尖吻鲈鱼鳞和鱼皮胶原蛋白的提取及其理化特性分析[J].食品科学,2018,39(1):36-41,6.基金项目
国家自然科学基金地区科学基金项目(31260376) （31260376）
