食品科学2018，Vol.39Issue(1)：58-64,7.DOI:10.7506/spkx1002-6630-201801009
百里醌对阪崎克罗诺肠杆菌的抑制作用
Antimicrobial Activity of Thymoquinone against Cronobacter sakazakii
摘要
Abstract
The objective of the present study was to evaluate the effectiveness of thymoquinone (TQ) against Cronobacter sakazakii strains.The minimum inhibitory concentration (MIC) of TQ against C.sakazakii and its effect on microbial growth kinetics were determined.Changes in intracellular ATP concentration,intracellular pH (pHin),membrane potential and membrane integrity were measured to elucidate the possible antimicrobial mechanism.Cell morphology changes were also observed under a field emission scanning electron microscope.The results showed that the MICs of TQ against C sakazakii strains ranged from 0.3 to 0.6 mg/mL,and TQ exposure resulted in a longer lag phase and lower specific growth rate compared to the control.TQ affected the cell membrane permeability of C.sakazakii,as evidenced by a reduction in intracellular ATP concentration from 6.52 to 0.27 and 0.17 μtnol/L and a decrease in pHin from 5.69 to 5.22 and 4.99 at MIC and 2 × MIC,respectively.TQ at both tested concentrations caused 80％ and 22％ reduction in cell integrity,respectively.In addition,cells treated with TQ showed hyperpolarized cell membrane.Field emission scanning electron microscopy illustrated that C.sakazakii cells exposed to TQ exhibited a more wrinkled surface compared with the smooth surface of untreated cells.In conclusion,TQ exerts its antimicrobial action partly by causing excessive permeability of the cell membrane and changing cell morphology.These findings suggest that TQ has potential application in controlling C.sakazakii in foods.关键词
阪崎克罗诺肠杆菌/百里醌/细胞膜/膜电位Key words
Cronobacter sakazakii/thymoquinone/cell membrane/membrane potential分类
生物科学引用本文复制引用
石超,孙慧慧,孙正,郭都,张文婷,孙怡,陈怡飞,夏效东..百里醌对阪崎克罗诺肠杆菌的抑制作用[J].食品科学,2018,39(1):58-64,7.基金项目
“十二五”国家科技支撑计划项目(2015BAD16B08) （2015BAD16B08）
中央高校基本科研业务费专项资金项目(2452017146) （2452017146）
