食品科学2018，Vol.39Issue(1)：149-155,7.DOI:10.7506/spkx1002-6630-201801023
南瓜、山药、葛根和桑叶提取物复方对四氧嘧啶模型小鼠的降血糖作用
Hypoglycemic Effect of Combined Extract of Pumpkin, Yam, Kudzuvine Root, and Mulberry Leaves on Alloxan-Induced Diabetic Mice
摘要
Abstract
Objective:The objective of this research was to study the hypoglycemic effect of the combined extract of pumpkin,yam,kudzuvine root and mulberry leaves on alloxan-induced diabetic mice.Methods:A diabetic mouse model was induced by alloxan.Another 10 mice served as a normal group.The model diabetic mice were randomly divided into model group,positive group (acarbose,200 mg/kg mb) and low and high dose treatment groups (12 and 36 g dry raw material/kg mb).After intragastric administration for 4 weeks,we studied the effect of the combined extract on the body weight,food and water intake,fasting blood glucose level,glycogen,serum insulin,glycosylated serum protein (GSP),glycosylated hemoglobin (GHb) and antioxidant status of diabetic mice.Results:The major bioactive components of the combined extract were pumpkin polysaccharide,yam polysaccharide,and puerarin at levels of (0.93 ± 0.04),(1.01 ± 0.06),and (0.28 ± 0.01) g/100 g dry raw material,respectively.Compared with the model group,the body weight and glycogen content of mice in the intervention group were significantly improved,while the food and water intake,fasting blood glucose,GSP and GHb were decreased significantly.The insulin sensitivity was also improved.The hypoglycemic effect of the high dose group was better than that of the low dose group.Meanwhile,compared with the model group,the total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity in the liver of mice administered with the combined extract were increased,while the content of malondialdehyde (MDA) was decreased.Thus,it was suggested that oxidative stress in diabetic mice was improved by administration of the combined extract.Conclusion:The combined extract of pumpkin,yam,kudzuvine root and mulberry leaves can effectively reduce the blood glucose level of alloxan-induced diabetic mice and improve oxidative stress.关键词
降血糖/提取物复方/四氧嘧啶/氧化应激Key words
hypoglycemic/combined plant extract/alloxan/oxidative stress分类
轻工纺织引用本文复制引用
赵磊,王鑫,姜飞,王成涛,徐宝财,王文侠..南瓜、山药、葛根和桑叶提取物复方对四氧嘧啶模型小鼠的降血糖作用[J].食品科学,2018,39(1):149-155,7.基金项目
科技创新服务能力建设-科技成果转化-提升计划项目(PXM2016-014213-000034) （PXM2016-014213-000034）
北京市科技计划项目(Z171100002217019) （Z171100002217019）
国家自然科学基金面上项目(31571801) （31571801）
