食品科学2018，Vol.39Issue(2)：31-37,7.DOI:10.7506/spkx1002-6630-201802006
辣木叶黄酮结构分析及其对胰脂肪酶的抑制作用
Structural Analysis and Anti-Pancreatic Lipase Activity of Flavonoids from Moringa oleifera Lam.Leaves
摘要
Abstract
The structure of flavonoids from Moringa oleifera Lam.leaves (FML) was analyzed.Meanwhile,the inhibitory effect of FML on pancreatic lipase was determined and the type of inhibition was investigated.The extraction yield of flavonoids reached (5.53 ± 0.11)％ with microwave-assisted extraction using 70％ ethanol as the extraction solvent.The obtained crude extract was purified by polyamide column chromatography and freeze-dried to obtain a powder.The total flavonoid content of the powder was (661.10 ± 9.20) mg/g.A total of 11 flavonoids were identified from FML by ultra performance liquid chromatography-quadrupole/electrostatic field orbital trap mass spectrometry (UPLC-Q-Orbitrap MS).The inhibitory activity of FML on pancreatic lipase was determined with p-nitrophenyl butyrate as substrate.The results showed that the purified FML had a strong inhibitory effect on pancreatic lipase,with an IC50 of 0.94 mg/mL,and the type of inhibition was determined as non-competitive inhibition by the Lineweaver-Burk double reciprocal plot method.关键词
辣木叶黄酮/超高效液相色谱-四极杆/静电场轨道阱质谱/胰脂肪酶/抑制作用Key words
flavonoids from Moringa oleifera Lam.leaves/UPLC-Q-Orbitrap MS/pancreatic lipase/inhibitory effect分类
轻工纺织引用本文复制引用
王远,郑雯,蔡珺珺,袁田青,宫兴文..辣木叶黄酮结构分析及其对胰脂肪酶的抑制作用[J].食品科学,2018,39(2):31-37,7.基金项目
浙江省自然科学基金项目(LY12C19011) （LY12C19011）
食品科学与工程浙江省重中之重一级学科基金项目(JYTSP20141052) （JYTSP20141052）
