食品科学2018，Vol.39Issue(2)：93-98,6.DOI:10.7506/spkx1002-6630-201802015
1株具抑菌和抗氧化活性乳酸菌的筛选及鉴定
Screening and Identification of Lactic Acid Bacterium with Antimicrobial and Antioxidant Activity
摘要
Abstract
In this study,lactic acid bacteria were isolated from pickle samples.All the isolates were primarily screened for their antimicrobial activity against six indicator strains such as Lactobacillus plantarum PL2 and Escherichia coli As 1.184.Then the scavenging activities against hydroxyl radicals,superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were measured to evaluate antioxidant activity of the isolates with antimicrobial activity.The results showed that eight strains with both antimicrobial and antioxidant activity were screened from pickles.The highest levels of antimicrobial activity and antioxidant activity were recorded in strain b-2,which was identified as L.plantarum by its physiological,biochemical and molecular characteristics.According to the results of antioxidant activity,the cell-free extract of b-2 showed the highest hydroxyl radical-scavenging activity,which scavenged 67.6％ hydroxyl radical,while the intact cells exhibited the highest superoxide anion-scavenging activity,which scavenge 62.8％ superoxide anion radical,and the highest DPPH radicalscavenging activity was seen in the cell-free supematant,which scavenged 91.3％ DPPH radical.To conclude,L.plantarum b-2 has good bacteriostatic ability and antioxidant capacity,and holds great potential as a functional lactic acid bacterial starter.关键词
抗氧化/抑菌/乳酸菌/筛选/鉴定Key words
antioxidant activity/antimicrobial/lactic acid bacteria/screening/identification分类
轻工纺织引用本文复制引用
张香美,赵玉星,闫晓晶,崔娜..1株具抑菌和抗氧化活性乳酸菌的筛选及鉴定[J].食品科学,2018,39(2):93-98,6.基金项目
国家自然科学基金面上项目(31471707) （31471707）
河北省高等学校科学技术研究项目(ZD20131091) （ZD20131091）
河北经贸大学2016年度大学生创新训练计划项目(201611832041) （201611832041）
