食品科学2018，Vol.39Issue(2)：138-143,6.DOI:10.7506/spkx1002-6630-201802022
三文鱼基质上单核细胞性李斯特菌毒力因子的表达
Virulence Gene Expression of Listeria monocytogenes Grown in Salmon Matrix
摘要
Abstract
The aim of the present study was to assess the influence of storage temperature on virulence gene expression of Listeria monocytogenes (Lm) grown in a food matrix.Lm ATCC 19115 was grown in a salmon matrix or tryptone soya agar containing 0.6％ yeast extract (TSA-YE) (control) at different temperatures (0,4 and 7 ℃) for 24 and 48 h,respectively.Then,the bacterial cells were collected by centrifugation for RNA extraction using a commercial kit (TianGen,Dalian),and the expression levels of hlyA,inlB,prfA and sigB were assessed by RT-qPCR.The RT-qPCR data showed that the expression of all four major virulence genes was up-regulated when the strain was grown in a salmon matrix compared to TSA-YE at each temperature.In addition,hlyA possessed the highest expression level among these four genes.The expression levels of prfA and sigB in Lm from salmon stored at 4 ℃ were significantly higher than those at 0 and 7 ℃ (P ＜ 0.01).The expression levels of inlB and hlyA in salmon stored for 24 h at different temperatures were in the descending order of 7 ℃ ＞ 4 ℃ ＞ 0 ℃ (P ＜ 0.01).In the salmon matrix,the expression levels of all four major virulence genes at 48 h were significantly higher than those at 24 h (P ＜ 0.01).The results of this study can provide basic information for risk assessment and control of Lm in food matrices.关键词
单核细胞性李斯特菌/毒力因子/三文鱼基质Key words
Listeria monocytogenes/virulence genes/salmon matrix分类
轻工纺织引用本文复制引用
朱雅慧,朱洪日,王佳莹,张公亮,郝洪顺,侯红漫..三文鱼基质上单核细胞性李斯特菌毒力因子的表达[J].食品科学,2018,39(2):138-143,6.基金项目
辽宁省自然科学基金项目(201602049) （201602049）
“十二五”国家科技支撑计划项目(2015BAD17B00) （2015BAD17B00）
