食品科学2018，Vol.39Issue(2)：254-259,6.DOI:10.7506/spkx1002-6630-201802040
超声辅助酶法提取中华鳖裙边胶原蛋白及其热稳定性能
Ultrasonic-Assisted Enzymatic Extraction and Thermal Stability of Collagen from Soft-Shelled Turtle Calipash
摘要
Abstract
In the current study we optimized the ultrasonic-assisted enzymatic extraction of collagen from soft-shelled turtle calipash.One-factor-at-a-time method was used to investigate four factors affecting the extraction efficiency including enzyme dosage,ultrasonic time,ultrasonic power and solid-to-solvent ratio.Subsequently,a mathematical model with enzyme dosage,ultrasonic time and ultrasonic power as independent variables was established by Box-Behnken design.Meanwhile,we compared the thermostability of collagen extracted by routine enzymatic extraction and ultrasoundassisted enzymatic extraction.The optimal extraction conditions that provided the maximum collagen yield (74.50％) were determined as follows:0.5 mol/L acetic acid as the extraction solvent,solid-to-liquid ratio 1∶20 (g/mL),enzyme dosage 0.8％,ultrasonic time 43 min,and ultrasonic power 176 W.The results of differential scanning calorimetry showed that the thermostability of collagen extracted by the modified method was better than that of collagen prepared by conventional enzymatic extraction.The results of this study can provide a basis for intensitive processing and application of collagen from soft-shelled turtle calipash.关键词
中华鳖裙边/超声辅助酶提优化/胶原蛋白/得率/热稳定性Key words
soft-shelled turtle calipash/optimization of ultrasonic-assisted enzymatic extraction/collagen/yield/thermal stability分类
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邹烨,蔡盼盼,王立,王道营,周涛,徐为民..超声辅助酶法提取中华鳖裙边胶原蛋白及其热稳定性能[J].食品科学,2018,39(2):254-259,6.基金项目
江苏省苏北科技专项(BN2015148) （BN2015148）
江苏省博士后科研资助计划项目(1601131C) （1601131C）
