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微波辅助水酶法优化红枣中环磷酸腺苷(cAMP)提取工艺及其抗疲劳作用

任彦荣 张忠明 谢跃杰 熊政委 王仲明 吴洪斌

食品科学2018,Vol.39Issue(2):260-266,7.
食品科学2018,Vol.39Issue(2):260-266,7.DOI:10.7506/spkx1002-6630-201802041

微波辅助水酶法优化红枣中环磷酸腺苷(cAMP)提取工艺及其抗疲劳作用

Optimization of Microwave-Assisted Aqueous Enzymatic Extraction of Cyclic Adenosine 3',5'-Monophosphate (cAMP) from Chinese Jujube (Ziziphus jujuba Mill.) and Its Anti-Fatigue Effect

任彦荣 1张忠明 2谢跃杰 3熊政委 1王仲明 1吴洪斌2

作者信息

  • 1. 重庆第二师范学院生物与化学工程学院,重庆400067
  • 2. 重庆第二师范学院重庆市功能性食品协同创新中心,重庆400067
  • 3. 甘肃农业大学食品科学与工程学院,甘肃兰州737100
  • 折叠

摘要

Abstract

Response surface methodology was used to optimize the microwave-assisted aqueous enzymatic extraction of cyclic adenosine 3',5'-monophosphate (cAMP) from Chinese jujube (Zizyphusjujuba Mill.) grown in Xinjiang.The antifatigue effect of the cAMP extracted was investigated.The optimal extraction conditions was found as follows:enzyme concentration 1.5% (a mixture of cellulase,hemicellulase and pectinase,1∶1∶1 (m/m)),temperature 31.3 ℃,time 20 min and microwave power 500 W.Under these conditions,the yield of cAMP was 560.9 μg/g.The results showed that the cAMP could extend exhaustive swimming time and enhance swimming endurance in mice,suggesting good anti-fatigue effect.Therefore,cAMP holds great promise for its application in the food and pharmaceutical industry.

关键词

红枣/环磷酸腺苷(cAMP)/微波辅助水酶法提取/游泳耐力

Key words

Zizyphus jujuba/cyclic adenodsine 3',5'-monophosphat (cAMP)/microwave-assisted aqueous enzymatic extraction/swimming time

分类

轻工纺织

引用本文复制引用

任彦荣,张忠明,谢跃杰,熊政委,王仲明,吴洪斌..微波辅助水酶法优化红枣中环磷酸腺苷(cAMP)提取工艺及其抗疲劳作用[J].食品科学,2018,39(2):260-266,7.

基金项目

新疆建设兵团工业科技攻关项目(2015AB030) (2015AB030)

重庆市五大功能区域创新创业团队建设项目(201618793) (201618793)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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