食品科学2018，Vol.39Issue(2)：273-280,8.DOI:10.7506/spkx1002-6630-201802043
铁皮石斛多糖提取工艺优化及对果蝇抗氧化能力的影响
Optimization of Extraction of Polysaccharide from Dendrobium officinale and Its Antioxidant Effect on Drosophila melanogaster
邱现创 1赵宁 1李晨 1王景雪1
作者信息
- 1. 山西大学生命科学学院,山西太原 030006
- 折叠
摘要
Abstract
The ultrasonic-assisted extraction of polysaccharide from Dendrobium officinale at a 250 W low power was optimized by response surface methodology based on central combination design.Extraction temperature,solvent-to-solid ratio,and ultrasonic treatment time were considered as independent variables.The optimum extraction conditions were determined as follows:extraction temperature 41.74 ℃2,solvent-to-solid ratio 50.26∶1 (mL/g),and ultrasonic treatment time 28.65 min.Under these conditions,the maximum extraction yield of 25.39％ was obtained,which was increased by 18％ compared with the value obtained using conventional hot water extraction with a simultaneous reduction in extraction time of 75％.Moreover,the DPPH and hydroxyl radical scavenging activities of polysaecharide from D.officinale were not reduced during ultrasonic-assisted extraction.Drosophila melanogaster were fed with different doses of the extracted polysaecharide,and the results showed that the polysaccharide at moderate dose provided the best antioxidant activity as evidenced by a 30％ increase in superoxide dismutase (SOD) and catalase (CAT) activity and a 46％ decrease in malondialdehyde (MDA) content of male D.melanogaster,as well as an increase of 60％ in CAT activity and 6％ in SOD activity and a 20％ decrease in MDA content of female D.melanogaster,extending the lifespan by an average of 20％.关键词
铁皮石斛/多糖/超声波/响应面优化/抗氧化/果蝇Key words
Dendrobium officinale/polysaccharide/ultrasonic/response surface analysis/antioxidant activity/Drosophila melanogaster分类
轻工纺织引用本文复制引用
邱现创,赵宁,李晨,王景雪..铁皮石斛多糖提取工艺优化及对果蝇抗氧化能力的影响[J].食品科学,2018,39(2):273-280,8.
