食品科学2018，Vol.39Issue(2)：287-292,6.DOI:10.7506/spkx1002-6630-201802045
应用Illumina Miseq测序分析饮用水源水中病毒多样性
Analysis of Virus Diversity in Drinking Source Water by Using Illumina MiSeq Sequencing Technology
摘要
Abstract
The viruses in drinking source water from east Tai Lake were separated and concentrated by gradient-series connection-circulation-tangential flow ultrafiltration (GSC-TFF).The viral genome was amplified by sequence independent single primer amplification (SISPA),sequenced by Illumina Miseq,and compared with the NCBI gene database using the basic local alignment search tool (BLAST).After quality control,1 190 914 928 bp gene data were obtained,which could be assembled into 5 554 scaffold sequences.Viral genome function was found and annotated to Caudovirales,Herpesvirales and Ligamenvirales at the family level.A total of 40 families of homologous sequences were annotated,Microviridae (27.590 1％),Siphoviridae (23.010 7％),Phycodnaviridae (5.322 2％),Retroviridae (1.691 2％),Mimiviridae (1.960 8％) being the dominant ones.A total of 102 112 reads (21.572 8％) were identified as no rank at the family level.The approach proposed in this study can allow high throughput analysis of virus diversity in source water,paving the foundation for detecting virus in water.关键词
Illumina Miseq测序/饮用水源水/病毒/多样性Key words
Illumina Miseq sequencing/drinking source water/virus/diversity分类
生物科学引用本文复制引用
葛英亮,于水利,时文歆..应用Illumina Miseq测序分析饮用水源水中病毒多样性[J].食品科学,2018,39(2):287-292,6.基金项目
“十二五”国家科技重大专项(2012ZX07403-001) （2012ZX07403-001）
