中国食品添加剂Issue(1)：187-193,7.
茚三酮比色法测定秋葵中氨基酸含量条件的优化
Optimization of detection conditions of amino acids in Okra by ninhydrin colorimetric method
摘要
Abstract
Optimization of detection condition of amino acids in Okra by ninhydrin colorimetric method and effect of different influence factors on detection were studied.The optimum extraction conditions of amino acids in Okra were determined by single factor and orthogonal test.The optimum detection conditions were:wavelength 560 nm,the ninhydrin (2％) volume 3mL,pH 6,heating temperature 100 ℃ and heating time 30 min.The optimum extraction conditions were:solid liquid ratio 1 ∶ 95,extraction time 10 rain,ultrasonic temperature 40 ℃,Under the above conditions,the extraction yield was 60.52 mg/g.The RSD of amino acids' content in Okra detected by this method was 1.12％ and the recovery was higher than 90％.The results showed that ninhydrin colorimetdc method was simple,sensitive,rapid,accurate,reliable and reproducible.It was suitable for detection of amino acid content in Okra.关键词
秋葵/氨基酸/茚三酮比色法Key words
Okra/amino acids/ninhydrin colorimetric method分类
轻工纺织引用本文复制引用
刘长姣,杨越越,王妮,余平..茚三酮比色法测定秋葵中氨基酸含量条件的优化[J].中国食品添加剂,2018,(1):187-193,7.基金项目
吉林省教育厅“十二五”科学技术研究项目(2014439). （2014439）
