中国食品学报2017，Vol.17Issue(10)：44-50,7.DOI:10.16429/j.1009-7848.2017.10.007
胶原蛋白与胶原蛋白肽的抗氧化关系初探
Preliminary Antioxidant Relationship of Collagen and Collagen Peptides
摘要
Abstract
The antioxidant activity of collagen have not been reported clearly,however the antioxidant activity of its hydrolysate collagen peptides have been reported frequently.In order to study the essential reason causing the difference,the antioxidant activity of collagen mimetic peptides,collagen peptides mixture,and its composing amino acid (Proline,Hydroxyproline and Glycine) was measured by T-AOC,hydroxylamine hydrochloride,DPPH and ABTS free radical methods;and the secondary structure of CMP and CP determined by circular dichroism.It was found that:1) The CMP,CP and various amino acid didn't exhibit antioxidant activity in the T-AOC method;2) Only CP showed detectable antioxidant activity in hydroxylamine hydrochloride method;3) CP,CMP and amino acids all had substantial antioxidant activity in DPPH and ABTS methods,with an order of CP＞Hypro＞CMP≥ Pro＞Gly;4) Circular dichroism data showed that CMP had high content of α-helix,which is necessary for the stable triple helix structure of collagen;however CP hadn't apparently α-helix structure.These results suggest that:1) The stable triple helix structure of collagen may lead to its antioxidant activity inconspicuously,and the antioxidant activity of collagen peptide relies on the connection of amino acid residues by peptide bonds and reachability of the side-chain of amino acid to free radical;2)The sensitivity of different antioxidant measurement for collagen peptide related molecules is variable,in which DPPH and ABTS method is relatively higher.关键词
胶原蛋白/胶原蛋白肽/抗氧化/3股螺旋结构Key words
collagen/collagen peptide/antioxidant/triple helix structure引用本文复制引用
林春通,柯欣欣,郭建辉,巫启明,刘树滔,郭静科,饶平凡,陈晓超..胶原蛋白与胶原蛋白肽的抗氧化关系初探[J].中国食品学报,2017,17(10):44-50,7.基金项目
国家自然科学基金项目(31071497,31271859,31500685) （31071497,31271859,31500685）
国家重点研发计划专项(2016YFD0400200) （2016YFD0400200）
浙江省自然科学基金项目(LY16C050002) （LY16C050002）
