中国食品学报2017，Vol.17Issue(12)：26-34,9.DOI:10.16429/j.1009-7848.2017.12.004
双孢菇内生枯草芽孢杆菌AB154中抑制人结肠癌Caco-2活性成分的发现及机理研究
The Anti-proliferation Activity and Mechanism of the Extract from Agaricus bisporus Mushroom Endophyte Bacillus subtilis AB154 on Human Colon Cancer Cell Line Caco-2
摘要
Abstract
A Bacillus subtilis AB154 with anti-proliferation activity on human cancer cells was isolated from the fruit body of white button mushroom (Agaricus bisporus),when we studied the anti-tumor activity of A.bisporus mushroom.The peptide with proliferation inhibiting activity produced by endophyte Bacillus subtilis AB154 was extracted using ammonium sulfate precipitation and DEAE-Sephadex ion exchange chromatography,followed by anti-tumor activity investigation.The results showed that it had a better inhibitory activity against the human colon cancer cell line Caco-2 with IC50 value of 109.94 μg/mL,while the inhibitory ability on human normal bronchial epithelial cell line NHBE was the lowest.Then a peptide component with anti-tumor ability was purified by the high performance liquid chromatography (HPLC),and its structure was identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and electro spray ionization tandem mass spectrometry (ESI-MS/MS).The result showed that the peptide produced by Bacillus subtilis AB154 belonged to the lipopeptide family of fengycin B1.This research first reported an fengycin B1 lipopeptide with anti-human colon cancer cell Caco-2 activity.The study of inhibitory mechanism showed that the cell cycle of Caco-2 cells treated with fengycin for 12 h were arrested in the G0/G1 phase,and the cell cycle was arrested in the S phase at 24 h.The fragmentation of nuclear chromatin within Caco-2 cells was induced by fengycin.关键词
双孢菇/内生菌/枯草芽孢杆菌/丰原素/人结肠癌Caco-2细胞Key words
Agaricus bisporus/endophytic/Bacillus subtilis/fengycin/human colon cancer cell line Caco-2引用本文复制引用
刘灿,生吉萍,David Yue,Wei Lee,李小可,尚小雅,陈林,申琳..双孢菇内生枯草芽孢杆菌AB154中抑制人结肠癌Caco-2活性成分的发现及机理研究[J].中国食品学报,2017,17(12):26-34,9.基金项目
公益性农业行业科研专项(200803033) （200803033）
