中国食品学报2017，Vol.17Issue(12)：116-121,6.DOI:10.16429/j.1009-7848.2017.12.015
固定化脂肪酶转酯化合成维生素A棕榈酸酯的分离纯化
Isolation and Purification of Retinol Palmitate Synthesized by Immobilized Lipase Transesterification
摘要
Abstract
The paper studied the isolation and purification of retinol palmitate synthesized by immobilized lipase with retinyl acetate and palmitic acid as substrates.Higher purity retinol palmitate was obtained using freezing crystallization and extraction.The technological processes were as follows:firstly,the reaction solution was placed at 4 ℃ for 20 h and then changed the temperature to-20 ℃ for another 8 h to get rid of the excessive palmitic acid.Then,the liquid-liquid extraction was done for further purification.The extraction solvent was 84％ ethanol/distilled water:petroleum ether (3:1),the extraction temperature was 4 ℃,the extraction cycle was 4.After extraction,the purity of retinol palmitate reached 98.3％.This work provided technical basis for the production of retinol palmitate.关键词
冷冻结晶/萃取/维生素A棕榈酸酯/分离纯化Key words
freezing crystallization/extraction/retinol palmitate/isolation and purification引用本文复制引用
董丛丛,黄高凌,杨远帆,李利君,陈昭华,杜希萍..固定化脂肪酶转酯化合成维生素A棕榈酸酯的分离纯化[J].中国食品学报,2017,17(12):116-121,6.基金项目
国家自然科学基金项目(31371751,31271914) （31371751,31271914）
福建省自然科学基金项目(2011J01225) （2011J01225）
