中国食品学报2018，Vol.18Issue(1)：243-249,7.DOI:10.16429/j.1009-7848.2018.01.031
抑制型表面等离子体共振免疫传感器再生循环检测花生中黄曲霉毒素B1
Detection of Aflatoxin B1 in Peanut by a Reproducible Surface Plasmon Resonance Immunosensor Based on Inhibition Format
摘要
Abstract
This study has developed a reproducible surface plasmon resonance (SPR) immunosensor based on the inhibition format for the detection of highly toxic pollutant-aflatoxin B1 (AFB1) in peanut.Methods:The antigen of AFB1 (AFB1-OVA) was prepared by mixed anhydride method,and pre-coated onto the surface of SPR chip using EDC/NHS.Based on the principle of immune suppression,the antibody for AFB,at a stable concentration was simultaneously bound by the target AFB1 and the coating antigen AFB1-OVA.According to the SPR response value obtained under different AFB1 concentrations,the inhibition ratio was calculated and the accurate and sensitive analysis of AFB1 in food could be realized according to the inhibiting ratio curve.Results:The concentration of AFB1 antibody was established at 80 nmol/L.Under the optimized experimental concentrations,the detection limit (IC15) and sensitivity (IC50) for AFB1 using the proposed SPR immunosensor achieved to 0.0049 μg/L and 0.025 μg/L.In chosen peanut sample,the detection limit (IC15) and sensitivity (IC50) were 0.13 μg/kg and 0.74 μg/kg,respectively.The whole detection process could be finished within 5 min,and one immune chip could be reused more than 60 times.Conclusions:The developed SPR immunosensor has offered a simple,fast and low-cost detection strategy with high sensitivity for AFB1,which can meet the requirements of rapid,accurate and cheap analysis of high toxicity AFB1 in food samples.关键词
黄曲霉毒素B1/表面等离子体共振/免疫传感器/可再生使用Key words
aflatoxin B1/surface plasmon resonance/immunosensor/reproducible引用本文复制引用
潘明飞,李诗洁,杨晶莹,徐乐灵,崔晓媛,王硕..抑制型表面等离子体共振免疫传感器再生循环检测花生中黄曲霉毒素B1[J].中国食品学报,2018,18(1):243-249,7.基金项目
天津市自然科学基金项目(17JCQNJC14800) （17JCQNJC14800）
天津市教委科研计划项目(2017KD01) （2017KD01）
中国大学生创新创业训练计划项目(201710057082) （201710057082）
