食品工业科技2017，Vol.38Issue(23)：85-91,7.DOI:10.13386/j.issn1002-0306.2017.23.018
响应面法优化酶法提取蜜环菌多肽及其抗疲劳活性
Optimization of enzymatic hydrolysis of Armillaria mellea Vahl exFr peptides by response surface methodology and its anti-fatigue ability
摘要
Abstract
Armillaria mellea Vahl exFr was obtained by enzymatic hydrolysis with Armillaria mellea Vahl exFr powder as raw material.The preparation process of walnut peptide was optimized on foundation of single factor experiment,according to Box-behnken central composition design principle,the method of response surface analysis was adopted with the degree of hydrolysis as the response value.The effect of concentration of substrate,enzyme dosage,time and temperature on the degree of hydrolysis were studied in this paper.Fatigue model was established by swimming test.Effects of Armillaria mellea Vabl exFr Peptides on swimming time,liver glycogen,muscle glycogen,serum urea nitrogen,lactic acid,LDH were determined.The rusults showed that the optimum preparation conditions were obtained as follows:concentration of substrate 7.4％,enzyme dosage 2％,time 4.6 h and temperature 51 ℃,the degree of hydrolysis was 35.61％,which was close to the theoretical value.Take the middle dose group as an example,compared to the blank group,the Armillaria mellea Vabl exFr Peptides increased content of liver glycogen and muscel glycogen 109.01％ and 58.33％,reduced metabolic product of LA and BUN levels 36.99％ and 30.33 ％,increased LDH activities 9％,their differences were significant (p ＜ 0.05).The Armillaria mellea ·Vahl exFr Peptides has the function of anti-fatigue obviously.关键词
蜜环菌/响应面/抗疲劳/多肽Key words
Armillaria mellea Vahl exFr/response surface methodology/anti-fatigue/peptide分类
轻工纺织引用本文复制引用
于欢,李露,王思爽,马丽颖,刘秋爽,钟炳昌,张慧锋..响应面法优化酶法提取蜜环菌多肽及其抗疲劳活性[J].食品工业科技,2017,38(23):85-91,7.基金项目
吉林省教育厅“十三五”科学技术研究项目(吉教科合字[2015]第379号) （吉教科合字[2015]第379号）
吉林医药学院大学生创新训练项目(2016049). （2016049）
