食品工业科技2017，Vol.38Issue(23)：104-109,6.DOI:10.13386/j.issn1002-0306.2017.23.021
卡拉胶酶的发酵培养基及培养条件优化
Optimization of medium composition and culture conditions for carrageenase production
摘要
Abstract
Biomass and carrageenase activity was used as the index,the effects of medium compositon and culture conditons on cell growth and enzyme production was investigated in shaking culture of P.carrageenovora ASY5.In order to increase the yield of carrageenase,the medium compositon and culture conditons were optimized for fermentation of Pseudoalteromonas carrageenovora ASY5.The optimal medium compostion was composed of carrageenan 5 g/L,tryptone 5 g/L,NaCl20 g/L,CaCl2 0.2 g/L,NaH2PO4· 2H2O 1.3 g/L,Na2HPO4· 12H2O 3.8 g/L,while the optimal culture conditions were determined as tempreture 18 ℃,initial pH6.5,inculation volume 2％,liquid volume 70 mL Under the optimum fermentation process,the production capability of carrageenase by P.carrageenovora ASY5 was significantly improved.Compared to the basic technical contidtions,the biomass and enzyme activity at the optimum technique were increased by 80.4％ and 52.2％,respectively.关键词
卡拉胶酶/发酵培养基/培养条件/工艺优化/酶活Key words
carrageenase/medium composition/culture conditions/processoptimization/enzyme activity分类
轻工纺织引用本文复制引用
朱云鹏,李永幸,洪清林,倪辉,郭东旭,肖安风..卡拉胶酶的发酵培养基及培养条件优化[J].食品工业科技,2017,38(23):104-109,6.基金项目
福建省高校产学合作项目(2016N5008) （2016N5008）
福建省科技重大专项项目(2015NZ0001-1) （2015NZ0001-1）
国家海洋公益行业科研专项(201505033) （201505033）
福建省海洋高新产业发展专项(闽海洋高新[2016]08号)资助. （闽海洋高新[2016]08号）
