食品工业科技2017，Vol.38Issue(23)：119-123,5.DOI:10.13386/j.issn1002-0306.2017.23.024
重组聚半乳糖醛酸酶PGB的生化特征及其酶解产物活性分析
Biochemical characterization of the recombinant polygalacturonase PGB and the activity analysis of its enzymatic hydrolysis product
谌新然 1黄永生 2田康明 1金鹏 2董自星 1刘晓光 1路福平 1王正祥2
作者信息
- 1. 天津科技大学化工与材料学院生物化工系,天津300457
- 2. 天津科技大学生物工程学院与工业发酵微生物教育部重点实验室,天津300457
- 折叠
摘要
Abstract
To analyze the enzymatic properties of recombinant polygalacturonase PGB and to study the compositions and biological activities of pectin hydrolysate prepared by PGB.Polygalacturonase was prepared by shake flask fermentation and its enzymatic properties were investigated.Pectin was hydrolyzed by PGB under optimum conditions,followed by investigation of its compositions,and antioxidant and antibacterial activities.At the shake flask fermentation level,the activity of PGB reached 10790 U/mLIts optimum temperature and pH was 55 ℃ and 5.0,respectively.And it was quite stable at 40 ℃ or pH4.0 ～6.0.Mg2+ and Cu2+ enhanced its activity,while K+,Ca2+,Zn2+,Mn2+,Co2+,Fe3+ and Fe2+ had different inhibitory effects on its activity.The Km and Vmaxof PGB toward low-ester pectin was determined to be 0.756 mg/mL and 9.225 mg/(mL·min).After hydrolyzed by PGB,pectin hydrolysate was identified as galacturonic acid and oligogalacturonic acid with a polymerization degree of 2 to 4 by high performance anion exchange chromatography-pulsed amperometric detector (HPAEC-PAD).This oligomer significantly inhibited the growth of Staphylococcus aureus,and its minimum inhibitory concentration and antioxidant activity was 0.64 mg/mL and 6.125 U/mL,respectively.关键词
聚半乳糖醛酸酶/生化特征/果胶/寡聚半乳糖醛酸Key words
polygalacturonase/biochemical characterization/pectin/oligogalacturonic acid分类
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谌新然,黄永生,田康明,金鹏,董自星,刘晓光,路福平,王正祥..重组聚半乳糖醛酸酶PGB的生化特征及其酶解产物活性分析[J].食品工业科技,2017,38(23):119-123,5.
