食品工业科技2017，Vol.38Issue(23)：268-274,7.DOI:10.13386/j.issn1002-0306.2017.23.049
真菌毒素DON和ZEN的体外联合毒性评价
Combinative toxicity assessment of mycotoxins DON and ZEN in vitro
徐慧 1石慧 2张银志 3孙秀兰 3纪剑 4张入元4
作者信息
- 1. 宁波方太厨具有限公司,浙江宁波315336
- 2. 天津顶育咨询有限公司食品安全中心,上海201103
- 3. 江南大学食品科学与技术国家重点实验室,江苏无锡214122
- 4. 江南大学食品学院,江苏无锡214122
- 折叠
摘要
Abstract
BEL-7402 cells were chosen as the cell model in this study to evaluate the indiviual and combined toxicity of Deoxynivalenol (DON)and Zearalenone (ZEN).With the logarithmic phase of BEL7402 cells exposed to different concentrations of DON,ZEN and their mixture for 24 h,the rate of cell proliferation,reactive oxygen species (ROS) and calcium levels,cell apoptosis rate and apoptosis related genes expression were analyzed and the combined effect of the two toxins was evaluated.The results showed that the mycotoxin DON,ZEN could inhibit cell activity with an IC50 of 9.3 μg/mL and 27.2 μg/mL,lead to cell damage induced by oxidative stress and increase the intracellular levels of reactive oxygen species and calcium ion concentration.Proapoptotic gene p53 was signifigantly up-regulation and Bal-2 gene was down-regulated,induce cell apoptosis or necrosis,a variety of toxic index verified that the combined toxicity of these two toxins on cells have an additive effect.关键词
脱氧雪腐镰刀菌烯醇/玉米赤霉烯酮/联合毒性/体外毒性/加和作用Key words
deoxynivalenol/zearalenone/combined toxicity/in vitro toxicity/additive effect分类
轻工纺织引用本文复制引用
徐慧,石慧,张银志,孙秀兰,纪剑,张入元..真菌毒素DON和ZEN的体外联合毒性评价[J].食品工业科技,2017,38(23):268-274,7.
