食品工业科技2017，Vol.38Issue(23)：289-294,6.DOI:10.13386/j.issn1002-0306.2017.23.053
新疆灰枣叶黄酮对衰老模型小鼠抗氧化活性的影响
Effects of flavonoids from Xinjiang Zizyphus jujube cv. Huizao leaves on antioxidant activity in aging mice mode
摘要
Abstract
Objective:To study the antioxidant activity of flavonoids from jujube leaves extract in aging mice's heart liver and serum.Methods:flavonoids from Jujube leaves was extracted with ethanol,the content of total flavonoids in jujube leaves was determined and its antioxidant activity was determined.The aging mouse model induced by subcutaneous injection of D-galactose was orally administered with Jujube leaves extract.The content of malondiaidehyde (MDA),the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px)and total antioxidant capacity (T-AOC)in heart,liver and serum of the mice were assessed;do HE staining slice,observe the state of tissue cells.Results:The content of total flavonoids in leaves was (18.60 ± 1.06) mg/g,The IC50 values of DPPH free radicals,hydroxyl radicals,ABTS radicals,and superoxide anion radicals were 0.997,1.035,1.039,0.983 mg/mL,all with positive control group had significant difference;D-galactose could obviously reduce T-AOC as well as SOD and GSH-Px activities,and enhance MDA contents in mouse heart,liver and serum,there was significant difference between the blank control group and the model group.In contrast,flavonoids from Jujube leaves could significantly enhance T-AOC,and SOD and GSH-Px activities,and reduce MDA contents in serum,and cerebrum,heart and liver tissues of the aging mice,especially in serum;According to the observation of pathological,flavonoids of jujube leaf have antioxidation ability.Conclusion:flavonoids from Jujube leaves could improve the activities of endogenous antioxidant enzymes in aging mice.Therefore,it has good antioxidant and anti-aging effects.关键词
枣叶黄酮/D-半乳糖/小鼠/抗氧化Key words
flavonoids from Jujube leaves extract/D-galactose/mice/antioxidation分类
轻工纺织引用本文复制引用
周新萍,邹玲,吴翠云,周润,白红进..新疆灰枣叶黄酮对衰老模型小鼠抗氧化活性的影响[J].食品工业科技,2017,38(23):289-294,6.基金项目
浙江大学馥莉食品研究院基金项目(KY201404). （KY201404）
