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苋菜芽生长中相关酶及抗氧化活性的变化

熊茜 王春幸 王凯凯 孙朦 王鸿飞 邵兴锋 许凤

食品工业科技2017,Vol.38Issue(24):10-15,6.
食品工业科技2017,Vol.38Issue(24):10-15,6.DOI:10.13386/j.issn1002-0306.2017.24.003

苋菜芽生长中相关酶及抗氧化活性的变化

Changes of related enzymes and antioxidant activities in the growth of amaranth bud

熊茜 1王春幸 1王凯凯 1孙朦 1王鸿飞 1邵兴锋 1许凤1

作者信息

  • 1. 宁波大学食品科学与工程系,浙江宁波315211
  • 折叠

摘要

Abstract

Changes of related enzymes and antioxidant activities in the process of amaranth growth were explored.The amaranth seeds were treated with various concentrations of MeJA and the optimal concentration was determined according to the growth of amaranth bud and the content of amaranthin.During the cultivation of amaranth bud treated with optimal concentration,the germination rate,germination energy,germination index,vigor index were investgated.Then the amaranth bud were collected regularly (2,4,6 and 8 d) for the determination of root length,overall length,amaranth content,total phenolic contents,hydrogen peroxide(H2O2)content and antioxidant enzyme activities.The results showed that 100 μmol/L MeJA treatment was not good for seed germination,which inhibited the root length and overall length significantly.However,it enhanced the contents of amaranth and total phenolic when compared to the control group.It eliminated O2-· by improving the activity of SOD,and it could also reduce the H2O2 accumulation in amaranth bud by delaying the increase of POD activity and the decrease of CAT and APX activities.This study would provide a new method to increase the yield of natural amaranthin,and it also provides theoretical basis for the further study of changes of related enzymes and antioxidant activities in the process of amaranth growth.

关键词

苋菜芽/苋红素/茉莉酸甲酯/过氧化氢/抗氧化酶

Key words

amaranth bud/amaranthin/MeJA/H2O2/antioxidant enzymes

分类

轻工纺织

引用本文复制引用

熊茜,王春幸,王凯凯,孙朦,王鸿飞,邵兴锋,许凤..苋菜芽生长中相关酶及抗氧化活性的变化[J].食品工业科技,2017,38(24):10-15,6.

基金项目

国家自然科学基金青年科学基金项目(31301574) (31301574)

浙江省自然科学基金项目(Y16C200011) (Y16C200011)

浙江省科技厅项目(2014C02023) (2014C02023)

宁波市自然科学基金项目(2015A610273) (2015A610273)

宁波大学人才引进项目(ZX2012000031) (ZX2012000031)

宁波大学学科项目(xkl1344) (xkl1344)

宁波大学校科研基金项目(XYL14025) (XYL14025)

宁波大学学科项目(xkzsc1526). (xkzsc1526)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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