食品工业科技2017，Vol.38Issue(24)：65-68,74,5.DOI:10.13386/j.issn1002-0306.2017.24.013
表儿茶素在模拟生理条件下对人血清蛋白糖基化反应的影响
Effect of epicatechin on glycation reaction of human serum albumin in physiological conditions
摘要
Abstract
In order to reveal the effect of epicatechin on protein glycosylation in physiological conditions,human serum albumin and ribose were used to construct Maillard reaction model.Inhibition rate,molecular weight,thermal aggregation,pH,free amino group content and surface hydrophobicity were analyzed in this study.The results showed that epicatechin had an inhibitory effect on glycosylation of human serum albumin,and with the increase of concentration,the inhibition rate of glycosylation increased.When the concentration of epicatechin was 0.04 mol/L,the inhibition rate was close to 80％.The results of molecular weight determination of glycosylation products showed that the molecular of glycosylation products decreased and no polymer was produced after adding the inhibitor.Analysis of the properties of glycosylation products showed that the thermostability of the glycosylation products were poor.The pH,free amino group content and surface hydrophobicity of the glycosylation products increased with the increase of epicatechin concentration,which indicated that glycosylation reaction was inhibited,the properties of the glycosylation products had changed.Therefore,epicatechin can be used as an inhibitor of glycosylation reaction in physiological conditions.关键词
蛋白质/表儿茶素/糖基化产物/人血清蛋白/核糖Key words
protein/epicatechin/glycosylation products/human serum albumin/ribose分类
轻工纺织引用本文复制引用
刘光宪,祝水兰,周巾英,熊慧薇,幸胜平,付晓记,冯健雄..表儿茶素在模拟生理条件下对人血清蛋白糖基化反应的影响[J].食品工业科技,2017,38(24):65-68,74,5.基金项目
江西省农业科学院青年基金(2015CQN002) （2015CQN002）
江西省科技计划(20161BBF60134). （20161BBF60134）
