食品工业科技2017，Vol.38Issue(24)：75-78,83,5.DOI:10.13386/j.issn1002-0306.2017.24.005
自由基氧化对中国对虾肌原纤维蛋白的影响
Effects of free radical oxidation on Chinese Penaeus myofibrillar protein
摘要
Abstract
In this study,the changes of myofibrillar protein and its properties,including carbonyl content,dimeric tyrosine content,mercapto content and the content of myofibrillar protein were studied,which were caused by protein oxidation,as treated by free radical oxidative system.The hydrophobicity of myofibrillar protein was observed and the hydrolysis of the protein was observed by SDS-PAGE.The simulated oxidation was carried out by free radical oxidation system(including FeCl3 and ascorbic acid concentration of 0.1 mmol/L,hydrogen peroxide concentration of 0,1,5,10 mmol/L)and were oxidized 1,3 and 5 h.The results showed that the carbonyl content,dimeric tyrosine content and surface hydrophobicity of mycelial fibrils were significantly increased(p ＜ 0.05)with the increasing of oxidant concentration after oxidation of free radical oxidative system free thiol content decreased significantly(p ＜ 0.05).It can be seen from the SDS-PAGE gel that the higher the concentration of oxidant,the more obvious hydrolysis of the treated protein.It can be concluded that free radical oxidation affected the carbonyl content,dimeric tyrosine content,mercapto content,hydrophobicity and the hydrolysis of protein of myofibrillar protein.关键词
中国对虾/肌原纤维蛋白/蛋白质氧化/电泳Key words
Chinese Penaeus/myofibrillar protein/protein oxidation/electrophoresis分类
轻工纺织引用本文复制引用
李亮,柏韵,尚宏丽,王宁,成天乐,李敏..自由基氧化对中国对虾肌原纤维蛋白的影响[J].食品工业科技,2017,38(24):75-78,83,5.基金项目
辽宁省自然科学基金项目(2015020776) （2015020776）
辽宁省农业领域创新人才项目(2014037) （2014037）
2016年辽宁省大学生创新训练项目(201610160000060). （201610160000060）
