食品工业科技2017，Vol.38Issue(24)：84-88,94,6.DOI:10.13386/j.issn1002-0306.2017.24.017
两步酶法提取裂壶藻油的工艺优化
Optimization of two-step enzymatic extraction of oil from Schizochytrium sp.
摘要
Abstract
Two-step enzymatic hydrolysis was uesd to extract oil from Schizochytrium sp.and the extraction conditions were optimized.The optimal extraction processing of oil from Schizochytrium sp were explored by single factor and orthogonal experiments,and verification experiments was used to verify the result of orthogonal experiments.The optimal extraction conditions was obtained as follows:liquid/solid ratio 1∶7,neutral protease addition 9％,enzymatic hydrolysis temperature 45 ℃,enzymatic hydrolysis time 3 h,enzymatic hydrolysis pH6.5,alkaline protease addition 12％,enzymatic hydrolysis temperature 70 ℃,enzymatic hydrolysis time 6 h,enzymatic hydrolysis pH9.5.Under these conditions,the clear oil extraction ratio from Schizochytrium sp.reached 90.22％.Gas chromatography-mass spectrometry (GC-MS) analysis showed that thecontent of unsaturated fatty acids was 44.13％ and the DHA content was 32.06％.关键词
裂壶藻/两步酶解/油脂提取/工艺优化Key words
Schizochytrium sp./two-step enzymatic method/oil extraction/process optimization分类
轻工纺织引用本文复制引用
吴兵兵,荣辉,杨贤庆,李来好,戚勃,马海霞,杨少玲..两步酶法提取裂壶藻油的工艺优化[J].食品工业科技,2017,38(24):84-88,94,6.基金项目
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2014TS24) （2014TS24）
广东省科技计划项目(2014A010107019) （2014A010107019）
广东省农业标准化专项资金项目 （）
国家重点研发计划项目-生物产业共性技术标准研究项目(2016YFF0202300) （2016YFF0202300）
农业部水产品加工重点实验室开放基金项目(NYJG201407). （NYJG201407）
