食品工业科技2017，Vol.38Issue(24)：95-99,5.DOI:10.13386/j.issn1002-0306.2017.24.019
小红豆抗菌蛋白的分离纯化及其功能活性
Isolation and purification of an antifungal protein from small red bean and its functional properties
摘要
Abstract
This essay was aim to isolate,pufificate and partial characterize the proteins with antifungal activity from the small red beans.A protein with antifungal activity was isolated and purified from small red beans by the procedure containing extraction,ammonium sulfate precipitation,Affi-gel blue gel chromatography,ion-exchange chromatography,and gel filtration chromatography.SDS-PAGE was used to analysis its purity and molecular weight.The result showed that the antifungal protein isolated and purified from small red beans exhibited a molecular mass of 4 kDa in SDS-polyacrylamide gel electrophoresis.Antifungal activity experiment showed that the protein had a strong inhibitory activity towards the growth of Fusarium oxysporum from eggplant,muskmelon and bitter gourd.An antifungal protein with a molecular mass of 4 kDa was isolated from small red beans.It exerted inhibitory activity on Fusarium oxysporum and had a high dependence on the concentration of protein.关键词
小红豆/抗菌蛋白/分离纯化/抗真菌活性Key words
small red beans/antifungal protein/isolation and purification/antifungal activity分类
轻工纺织引用本文复制引用
赵立娜,何庆燕,汪少芸..小红豆抗菌蛋白的分离纯化及其功能活性[J].食品工业科技,2017,38(24):95-99,5.基金项目
国家自然科学基金面上项目(31571779) （31571779）
国家自然科学基金青年科学基金项目(31501432). （31501432）
