食品工业科技2017，Vol.38Issue(24)：99-103,5.DOI:10.13386/j.issn1002-0306.2017.24.020
杏鲍菇重组漆酶对不同酚类污染物的降解研究
Phenic pollution treatment using recombinant laccase from Pleurotus eryngii
摘要
Abstract
The enzymatic properties of recombined laccase derived from Pleurotus eryngii were investigated.The degradation effects of laccase on two kinds of phenols (nonylphenol and 2,4-dichlorophen) were evaluated through optimizing the degradation time,enzyme dosage,phenol concentration and pH.The optimal reaction temperature of recombined laccase was 60 ℃,while the optimal pH was 2.5～3.0.The recombined laccase could keep high thermal stability below 30 ℃ and high pH stability between 9.0～10.0.The recombined laccase could decompose phenols with different structures,but the degradation ratios of phenols by laccase were decreased with increasing concentrations of phenols.With degradation time of of 16 h,enzyme dosage of 1 U/mL,pH of 3.5,the degradation ratio of nonylphenol(10 mg/L)reached up to 100％;with degradation time of 16 h,enzyme dosage of 1.25 U/mL,pH of 4.0,the degradation ratio of 2,4-dichlorophen (5 mg/L)reached up to 95.1％.The study of recombined laccase derived from Pleurotus eryngii indicated a theoretical basis for the treatment of phenol-containing pollution.关键词
重组漆酶/壬基酚/2,4-二氯酚/降解Key words
recombinant laccase/nonylphenol/2,4-dichlorophenol/degradation分类
轻工纺织引用本文复制引用
夏文静,周学,周燕,李飞..杏鲍菇重组漆酶对不同酚类污染物的降解研究[J].食品工业科技,2017,38(24):99-103,5.基金项目
江苏省高校自然科学研究面上项目(15KJB220004) （15KJB220004）
江苏省高校自然科学研究面上项目(16KJB180017) （16KJB180017）
泰州市社会发展项目(TS201516) （TS201516）
南京师范大学泰州学院第二批精品课程《酶工程》. （）
