食品工业科技2017，Vol.38Issue(24)：109-113,119,6.DOI:10.13386/j.issn1002-0306.2017.24.022
酒醅中高产纤维素酶菌株的筛选及其酶学性质
Study on screening of high-yield cellulase strains in the fermented grains and its enzymatic properties
摘要
Abstract
Cellulase has broad application prospects,and has respective characteristics according to the different sources.The cellulase microbes were screened from Luzhou-flavor fermented grains,and further plan to use for the degradation and utilization of cellulose in distiller's grains.Sodium carboxymethyl cellulose culture medium containing Congo red was used to screen for producing enzyme strains,a highly cellulose-secreted strain XWS-1 was obtained.The strain was identified as Bacillus subtilis.The optimum conditions in 1％ CMC-Na medium were as follows:35 ℃,initial pH5.5,cultivate 72 h.The enzymatic activity properties of XWS-1 strains was further studied.The results showed that the optimum reaction temperature of crude enzyme fluid was 40 ℃,and the optimum reaction pH was 5.5.In summary,the cellulase produced by XWS-1 is a medium-temperature and acidic cellulase,which is suitable for the degradation of acidic cellulosesuch as distiller's grains.This work not only provide a foundation for degradation of cellulose in distiller's grains,but also provide a genetic material for recombinant complex cellulasethatcan using degradation of cellulose in distiller's grains.关键词
纤维素酶/分离鉴定/白酒酒醅/产酶特性/酶学性质Key words
cellulase/isolation and identification/fermented grains/properties of enzyme producing/characteristic of enzyme分类
轻工纺织引用本文复制引用
李永博,暴金磊,万敏,黄治国,任志强..酒醅中高产纤维素酶菌株的筛选及其酶学性质[J].食品工业科技,2017,38(24):109-113,119,6.基金项目
四川省教育厅重点项目(17ZA0272) （17ZA0272）
四川理工学院科研基金人才引进项目(2015RC45) （2015RC45）
四川省教育厅2016年省级大学生创新创业训练计划项目(201610622091). （201610622091）
