食品工业科技2017，Vol.38Issue(24)：217-221,5.DOI:10.13386/j.issn1002-0306.2017.24.042
水提铵沉法提取蜂王浆水溶性蛋白工艺优化
Optimization of water-soluble protein extraction process of royal jelly by water extraction and ammonium precipitation
摘要
Abstract
This experiment chose the royal jelly from Heilongjiang Mishan as raw material and took water-soluble protein extraction rate as evaluation indicator.Based on single factor experiment and Plackett-Burman design,the response surface optimization method was used to optimize the extraction of water-soluble protein from royal jelly by water extraction ammonium sulfate precipitation.The experiment showed that the optimum technological parameters were material water mass ratio 1 ∶ 20,ultrasonic extraction time 40 minutes,ammonium sulfate saturation 89％,pH5,precipitation time 50 minutes.Under the condition of this,the water-soluble protein extraction rate of royal jelly was up to 80.22％,this method of experiment was the better way to extract water-soluble protein of royal jelly.关键词
水提铵沉法/蜂王浆/水溶性蛋白/提取工艺/优化Key words
water extraction of ammonium sulfate precipitation method/royal jelly/water-soluble protein/extraction process/optimization分类
轻工纺织引用本文复制引用
张越,付莉..水提铵沉法提取蜂王浆水溶性蛋白工艺优化[J].食品工业科技,2017,38(24):217-221,5.基金项目
辽宁医学院校内课题(LYHX20120810). （LYHX20120810）
