食品工业科技2017，Vol.38Issue(24)：227-230,237,5.DOI:10.13386/j.issn1002-0306.2017.24.044
酰化高丝氨酸内酯对巨噬细胞模型影响初探
Preliminary study on the effect of acyl-homoserine lactones on macrophage model
摘要
Abstract
Acyl-homoserine lactones(AHLs) compound is a class of the most important signaling molecule in quorum sensing system of gram-negative bacterium,this paper simulates 5 dominant AHLs (C4-HSL,C6-HSL,C8-HSL,C10-HSL,and 3OC12-HSL) generated from gram negative bacteria in freshwater fish,and screens the macrophage strain with sensitive identification of specific AHLs by constructing two macrophage models of Ana-1 and RAW 264.7.The results showed that each of the 5 AHLs could cause the decrease of Ana-1 and RAW 264.7 cell activeness.Through the intracellular Ca2+ fluorescence detection,under C10-HSL stimulation,the activity of Ana-1 cells showed a certain correlation with intracellular Ca2+,it was speculated that C10-HSL could induce the intracellular Ca2+ increase in Ana-1 cells,thus trigger the fluorescence reaction of intracellular Ca2+ probe.Meanwhile the sensitivity of Ana-1 cells to C10-HSL was verified by flow cytometry and electron microscopy,which provided the basis and guidance for the construction of ANA-1 cell based fluorescent sensing method for detection of signaling molecule C10-HSL in freshwater fish spoilage organisms.关键词
淡水鱼腐败菌/信号分子/巨噬细胞/细胞凋亡Key words
spoilage bacteria of freshwater fish/quorum sensing signaling molecules/macrophages/cell apoptosis分类
轻工纺织引用本文复制引用
蒋栋磊,刘延,葛攀玮,吴满刚,葛庆丰,方维明..酰化高丝氨酸内酯对巨噬细胞模型影响初探[J].食品工业科技,2017,38(24):227-230,237,5.基金项目
国家自然科学基金面上科学基金项目(31601535,31271945,31371792) （31601535,31271945,31371792）
江苏省自然科学基金青年基金项目(BK20160459) （BK20160459）
江苏省高校自然科学研究面上项目(16KJB550008) （16KJB550008）
扬州市社会发展前瞻性研究项目(YZ2014177) （YZ2014177）
2017国家级大学生创新训练项目(201711117042Z) （201711117042Z）
2017年度江苏省扬州大学研究生实践创新计划(SJCX17_0631). （SJCX17_0631）
