食品工业科技2017，Vol.38Issue(24)：259-263,267,6.DOI:10.13386/j.issn1002-0306.2017.24.050
高效液相色谱-串联质谱法测定猪肉中氯霉素和五种磺胺类药物的残留量
Determination of residues of chloramphenicol and five sulfonamides in pork by ultra-high liquid chromatography-tandem mass spectrometry
摘要
Abstract
Objective:A method of determination of chloramphenicol,sulfamerazine,sulfadimidine,sulfathiazole,trimethoprim,and sulfadiazine in pork by ultra-high liquid chromatography-tandem mass spectromrtry (UPLC-MS/MS)was developed.Method:Samples were extracted with ethyl acetate precipitated protein at the same time and dried under nitrogen.The residue was dissolved with 10％ acetonitrile solution and defatted by cyclohexane.Samples were detected by UPLC-MS/MS and isolated with UPLC BEH C18.The liquid phase was acetonitrile and water.The electrospray ionization (ESI)mass spectrometry had run in multiple reaction monitoring mode (MRM).Result:At these experimental conditions,chloramphenicol had good liner relatively(R2 ＞ 0.99) between 9.6 ～ 384 ng/kg.And sulfamerazine,sulfadimidine,sulfathiazole,trimethoprim and sulfadiazine had good liner relatively(R2 ＞ 0.99) between 0.51 ～20.8 μg/kg.Limits of detection were 3.2 ng/kg for chloramphenicol,0.158～ 0.173 μg/kg for 5 kinds of sulfonamides (sulfamerazine,sulfadimidine,sulfathiazole,trimethoprim,sulfadiazine).Limits of quantitation were 9.6 ng/kg for chloramphenicol,0.475～0.52 μg/kg for 5 kinds of sulfonamides(sulfamerazine,sulfadimidine,sulfathiazole,trimethoprim and sulfadiazine).Average recoveries were 70.84％～ 103.99％ with relative standard deviations of 1.98％～8.01％.Conclusion:This method could detect the chloramphenicol,sulfamerazine,sulfadimidine,sulfathiazole,trimethoprim,and sulfadiazine residue in pork with a better precision,higher accuracy and better specificity.关键词
高效液相色谱-串联质谱法/猪肉/氯霉素/磺胺类药物Key words
UPLC-MS/MS/pork/chloramphenicol/sulfonamides分类
轻工纺织引用本文复制引用
陆苑,伍萍,杨淑婷,李勇军,兰燕宇,孙佳..高效液相色谱-串联质谱法测定猪肉中氯霉素和五种磺胺类药物的残留量[J].食品工业科技,2017,38(24):259-263,267,6.基金项目
贵州省高等学校创新能力提升计划(黔教合协同创新字[2013]04) （黔教合协同创新字[2013]04）
贵州省优青项目(黔科合人字[2015]11号) （黔科合人字[2015]11号）
现代药物研究开发协同创新中心(黔教合协同创新字[2013]04). （黔教合协同创新字[2013]04）
