食品工业科技2017，Vol.38Issue(24)：274-277,4.DOI:10.13386/j.issn1002-0306.2017.24.053
超高效液相色谱-串联质谱法测定运动饮品中的叶酸
Determination of folic acid in sports drinks by UPLC-MS/MS
摘要
Abstract
This paper used ultra high performance liquid chromatography tandem mass spectrometry analysis method to establish a method in the analysis of folic acid in sports drink.After the sample was treated with 10％ ethanol to extraction,the separation of targeted compound was performed on a T3 chromatographic column (2.1 mm × 150 mm,3 μm)using 10.0％ methanol-0.1％ formic acid as mobile phase combined with ultra performance liquid chromatography tandem mass spectrometry instrumentation.The linear range of folic acid was in the range of 2.0～ 100.0 ng/mL with a correlation coefficient of 0.9992.The result showed that the detection limit was 1.0 μg/kg and the quantitative limit was 3.0 μg/kg.The recovery rate was 91.9％ ～ 96.0％,and the relative standard deviation(RSD) was 2.6％ ～3.2％.The method has the advantages of simple pretreatment,high sensitivity and fast detection rate,and is suitable for determination of folic acid in a wide range for sports drinks,and can provide reference for its analysis.关键词
运动饮品/超高效液相色谱-串联质谱/叶酸Key words
sports drink/ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)/folic acid分类
轻工纺织引用本文复制引用
冀晔..超高效液相色谱-串联质谱法测定运动饮品中的叶酸[J].食品工业科技,2017,38(24):274-277,4.
