食品与机械2017，Vol.33Issue(9)：15-19,5.DOI:10.13652/j.issn.1003-5788.2017.09.004
外源性氧化胁迫对酿酒酵母突变株Y518合成谷胱甘肽的影响
Effects of exogenous oxidation stress on glutathione synthesis in Saccharomyces cerevisiae mutant Y518
郑丽雪 1齐斌 1孙姜 2王立梅1
作者信息
- 1. 常熟理工学院生物与食品工程学院,江苏常熟215500
- 2. 中海海洋无锡海洋工程装备有限公司,江苏无锡214000
- 折叠
摘要
Abstract
Effects of exogenous oxidative stress on glutathione production by Saccharomyces cerevisiae mutant Y518 were researched by addition of exogenous hydrogen peroxide.The results showed that the addition of hydrogen peroxide inhibited the growth of Y518 cells,but promoted the synthesis of glutathione of Y518.The best addition time and amount of hydrogen peroxide were respectively 24 h and 0.6 mmol/L.The content of gluconate reached (13.69±0.28) mg/g,increased by about 14 ％ compared to that without hydrogen peroxide.Exogenous oxidation stress on Y518 was produced by adding 0.6 mmol/L hydrogen peroxide at 24 h,which activated the transcription regulation factor YAP 1 and SKN7 to control expressions of γ-glutamylcysteine synthetase,then that of glutathione synthetase encoded genes GSH Ⅰ and GSH Ⅱ,and finally enhanced the activities of γ-glutamylcysteine and glutathione synthetases to promote glutathione synthesis in Y518.Therefore,the addition of exogenous hydrogen peroxide could produce exogenous oxidation stress on Y518 and further promote Y518 to synthesize glutathione.关键词
过氧化氢/酿酒酵母/氧化胁迫/谷胱甘肽Key words
hydrogen peroxide/Saccharomyces cerevisiae/oxidation stress/glutathione引用本文复制引用
郑丽雪,齐斌,孙姜,王立梅..外源性氧化胁迫对酿酒酵母突变株Y518合成谷胱甘肽的影响[J].食品与机械,2017,33(9):15-19,5.
