食品与机械2017，Vol.33Issue(9)：59-63,5.DOI:10.13652/j.issn.1003-5788.2017.09.012
3种海洋鱼油脂肪酸组成及其位置分布
Composition and positional distribution of fatty acids in triacylglycerols of three marine fish oils
张惠君 1王兴国 2金青哲3
作者信息
- 1. 江南大学食品学院,江苏无锡214122
- 2. 江南大学国家功能食品工程技术研究中心,江苏无锡214122
- 3. 江南大学江苏省食品安全与质量控制协同创新中心,江苏无锡214122
- 折叠
摘要
Abstract
In this study,the purified triacylglycerols (TAG) of selected marine fish oils (anchovy,tuna and salmon oils) were subjected to stereospecific hydrolysis by an sn-1,3-specific lipase (Lipozyme RM IM) which cleaves selectively the sn-1 and sn-3 position ester bonds of TAG yielding to sn-2-monoglycerides (2-MAG).After lipase hydrolysis,the released free fatty acids and sn-2-MAG were isolated by thin-layer chromatography (TLC).The original TAG and sn-2-MAG were respectively converted to fatty acid methyl esters (FAME),then analyzed by gas chromatography(GC).Results showed that TAG of these marine fish oils were made up of unsaturated fatty acids mainly accounting for 60％.Polyunsaturated fatty acids are preferentially located at sn-2 position,whose percentage is more than 50％,while saturated fatty acids and monounsaturated fatty acids are most distributed in sn-1,3 position.The ratio between n-3 and n-6 PUFAs is greater than 10,which is in line with the FAO/WHO recommended intake standard (＞ 4) and is ideal for n-3 PUFA natural nutritional supplements.关键词
甘三酯/米黑根毛霉源固定化脂肪酶/sn-2-单甘酯/薄层层析/气相色谱Key words
TAG/lipozyme RM IM/sn-2 monoglyceride/thin-layer chromatography/gas chromatography引用本文复制引用
张惠君,王兴国,金青哲..3种海洋鱼油脂肪酸组成及其位置分布[J].食品与机械,2017,33(9):59-63,5.
