食品与机械2017，Vol.33Issue(9)：151-155,5.DOI:10.13652/j.issn.1003-5788.2017.09.032
鲢鱼鱼精蛋白超声波辅助提取工艺优化及其抗菌活性研究
Optimization of extraction process assisted by ultrasonic and antibacterial activity evaluation on the protamine from silver carp
摘要
Abstract
To realize the high comprehensive utilization on the byproduct of silver carp,taking the testis sacs of silver carp as the raw material,the effects were studied,including the concentration of citric acid,the temperature,ultrasonic power,ultrasonic time,solid-liquid rate and extraction times on the efficacy extraction of silver carp spermary.On the base of single-factor experiments,and through the orthogonal test,the optimization experiment conditions were as followed:concentration of citric acid 0.45 mol/L,extraction temperature 55 ℃,the ultrasonic time 20 min,ultrasonic power 400 W,ratio of solid-liquid 1 ∶ 30,and the extraction times 3 times.The extraction ratio of chub protamine was 3.25％ at the optimized conditions.Four groups chub protamine were purified and separated through sepharose fast flow ion-exchanged chromatography.By studying the antibacterial activities of the four group,excepting the precursor protein,the other groups had a good inhibition on the salmonella,staphylococcus aureus and escherichia coli,especially the group Ⅱ and Ⅲ.关键词
鲢鱼/超声波辅助提取/鱼精蛋白/离子交换纯化/抑菌Key words
silver carp/ ultrasonic assisted extraction/protamine/ion-exchange chromatography/anti-bacteria引用本文复制引用
刘光宪,祝水兰,何家林,周巾英,冯健雄..鲢鱼鱼精蛋白超声波辅助提取工艺优化及其抗菌活性研究[J].食品与机械,2017,33(9):151-155,5.基金项目
江西省农业科学院青年基金(编号:2015CQN002) （编号:2015CQN002）
江西省科技计划(编号:20161BBF60134) （编号:20161BBF60134）
