现代食品科技2018，Vol.34Issue(1)：13-18,6.DOI:10.13982/j.mfst.1673-9078.2018.1.003
EGCG对高脂饮食诱导的肥胖HFA小鼠脂质代谢的影响
Effects of EGCG on the Lipid Metabolism in Obese HFA Mice Induced by High-Fat-Diet
摘要
Abstract
The lipid-lowering effects of (-)-epigallocatechin-3-gallate (EGCG) on obese human flora-associate (HFA) mice induced by high fat diet were investigated in this study.The aseptic Kunming (KM) mice were inoculated with healthy human excrement suspension to construct HFA model and randomly divided into two groups:high-fat group (40) and normal group (10) were fed with high-fat diet and normal diet,respectively.After 8 weeks,the mice were sacrificed and the body weight,liver weight,epididymal fat pad weigh,blood glucose,blood lipid and hematoxylin and eosin (HE) staining of liver and epididymal fat pad were measured.The obese mice were randomly divided into three groups:the control group,0.2％ EGCG low-dose group and 0.8％ EGCG high-dose group,and fed with normal diet After 8 weeks,the mice were sacrificed and several indices mentioned above were assayed.The results showed that the epidihymal fat pad weight,total cholesterol (Tch) and low density lipoprotein content (LDL-C) in high dose group were significantly decreased by 48.15％,28.64％ and 43.16％,respectively,as compared to the normal group.Furthermore,EGCG treatment also normalized the cellular morphology of the epidihymal fat pad,and alleviated hepatic damage in a dose-dependent manner.These results suggested that EGCG had certain effects on lipid-decreasing and liver injury repair activity in obese HFA mice.关键词
没食子儿茶素没食子酸酯/高脂饮食诱导人源菌群小鼠/肥胖/苏木精-伊红染色Key words
epigallocatechin gallate/human flora-associated mice/obesity/hematoxylin-eosin staining引用本文复制引用
胡凌,曾本华,王洁,董飒爽,廖振林,王丽,魏泓,方祥..EGCG对高脂饮食诱导的肥胖HFA小鼠脂质代谢的影响[J].现代食品科技,2018,34(1):13-18,6.基金项目
国家自然科学基金资助项目(31671855) （31671855）
广东省农业技术需求研究与示范项目(2016LM3179) （2016LM3179）
广东省海洋和渔业发展专项资金项目(粤财农[2017] 17号) （粤财农[2017] 17号）
