现代食品科技2018，Vol.34Issue(1)：221-226,194,7.DOI:10.13982/j.mfst.1673-9078.2018.1.034
微滴式数字PCR对肉制品中羊肉和猪肉定量分析
Quantitative Analysis of Mutton and Pork in Meat Products by Micro Digital PCR
摘要
Abstract
In order to realize the quantitative research of mutton and pork in meat products,a quantitative method based on the droplet digital polymerase chain reaction (ddPCR) was used for quantitative detection of meat products in this study.It was better to identify the adulteration of human factors or inadvertent bringing into during the process by designing single primers on housekeeping genes.The results of ddPCR showed that the relationships between the DNA concentration and DNA copy number(C) were close to be linear in a certain rage of meat weight and DNA concentration.This method was validated by artificial adulteration and commercial sample detection.The DNA concentration was utilized as an intermediate value to establish the formulae about the relationship between the original meat weight and DNA copy number:Movine=0.12C-10.6,Mpork=0.07C+4.By examining the mixed meat samples of known adulteration composition and commercially available samples,a more accurate method for detecting the adulteration meat was established.It shows that there is a certain adulteration in the market,and the droplet digital polymerase chain reaction(ddPCR) established in this paper has great potential in adulteration detection of mutton and pork and their products,which provides a scientific basis for the market supervision of adulterated meat products at present.关键词
微滴式数字PCR/肉类掺假/定量研究/羊肉/猪肉Key words
droplet digital PCR/meat adulteration/quantitative study/mutton/pork引用本文复制引用
陈晨,张岩,李永波,李鹏,李永艳,张涛,张薇,周巍,张志胜..微滴式数字PCR对肉制品中羊肉和猪肉定量分析[J].现代食品科技,2018,34(1):221-226,194,7.基金项目
科技部公益性行业科研专项(201510208-08) （201510208-08）
河北省科技计划项目(17275507D) （17275507D）
