食品科学2018，Vol.39Issue(3)：21-27,7.DOI:10.7506/spkx1002-6630-201803004
荞麦壳提取物有效组分的分离及体外抗糖尿病活性
Isolation and Anti-Diabetic Activity in Vitro of Flavonoids from Buckwheat Hull
摘要
Abstract
The purpose of this investigation was to evaluate the in vitro bioactivity of buckwheat hull extract (BHE). BHE was purified and separated into 5 fractions: BHE-M1, BHE-M2, BHE-M3, BHE-M4 and BHE-M5 by sequential chromatography on macroporous resins. The inhibitory activity against α-glycosidase and advanced glycosylation end products (AGEs) formation of these fractions was assessed. The results indicated that the purity of BHE was increased from 30.8 to 65.3 g/100 g after purification and BHE-M4 had the highest purity of 89.2 g/100 g among the five fractions. The percentage inhibition of α-glycosidase by BHE, purified BHE, BHE-M2, BHE-M3, BHE-M4 and BHE-M5 at a concentration of 1.000 mg/mL were 16.03%, 24.39%, 18.04%, 25.46%, 28.23% and 26.24%, respectively, which were significantly higher than that of the positive control arabinose. In glucose-bovine serum albumin (G-BSA) and fructose-bovine serum albumin (F-BSA) systems, all samples except BHE-M1 had a stronger inhibitory effect on AGEs formation than amino guanidine with BHE-M4 being the mwost effective. High performonce liquid chromatography-mass spectrometry (HPLC-MS) results indicated that flavonoids identified in BHE-M4 were kaempferol-3-O-β-D-glucoside or kaempferol-3-O-β-D-galactosidase, vitexin, rutin, isoquercitrin or hyperoside and quercitrin. Conclusively, flavonoid-rich extracts from buckwheat hull possessed a stronger inhibitory effect on α-glycosidase activity and AGEs formation, implying that flavonoids are the major antidiabetic components in buckwheat hull.关键词
荞麦壳/黄酮/大孔树脂/糖尿病/α-葡萄糖苷酶/蛋白质糖基化终末产物Key words
buckwheat hull/flavonoids/macroporous resin/diabetes mellitus/α-glycosidase/advanced glycosylation end products (AGEs)分类
轻工纺织引用本文复制引用
赵梓瀛,朴春红,王玉华,刘俊梅,于寒松,代伟长,唐玉芳,王婧,刘岱琳..荞麦壳提取物有效组分的分离及体外抗糖尿病活性[J].食品科学,2018,39(3):21-27,7.基金项目
国家自然科学基金青年科学基金项目(31201345) （31201345）
吉林省科技厅中青年科技创新人才及团队项目(20160519013JH) （20160519013JH）
