食品科学2018，Vol.39Issue(3)：176-181,6.DOI:10.7506/spkx1002-6630-201803027
葡萄籽原花青素提取物对砷诱导人肝细胞HL-7702损伤的保护作用及其机制
Protective Mechanism of Grape Seed Proanthocyanidin Extract against Oxidative Damage Induced by Arsenic in HL-7702 Cells
摘要
Abstract
Objective: To explore the protective mechanism of grape seed proanthocyanidin extract (GSPE) against oxidative damage induced by arsenic for the purpose of providing a theoretical foundation for the prevention and treatment of arsenic poisoning and for the evaluation of the antioxidant activity of GSPE. Methods: HL-7702 cells were randomly divided into seven groups: control, arsenic poisoning (25 μmol/L), GSPE intervention (5, 10, 25 and 50 mg/L), and single GSPE treatment (50 mg/L) groups. After treatment for 24 and 48 h, the levels of aspartate transaminase (AST), alanine aminotransferase (ALT), glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) were determined by commercial kits. Cell viability was tested by 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide (MTT). We used polymerase chain reaction (PCR) and Western blot to detect the mRNA and protein expression levels of nuclear factor erythroid-2 related factor 2 (Nrf2), glutathione S-transferase (GST), heme oxygenase 1 (HO-1), and quinone oxidoreductase 1 (NQO1). Results: The cell viability in the high-concentration arsenic groups (≥ 25 μmol/L) was significantly lower than that in the control group (P < 0.05). The levels of ALT, AST and MDA in the GSPE intervention group were significantly lower than those in the arsenic poisoning group (P < 0.05), while a significant elevation was observed for GSH, T-AOC and SOD (P < 0.05). The mRNA and protein expression levels of Nrf2, GST, HO-1 and NQO1 in the GSPE intervention group were significantly elevated when compared with the arsenic poisoning group (P < 0.05). Conclusion: GSPE has an antagonistic action against arsenic poisoning in a dose-dependent manner by activating the Nrf2-mediated signaling pathway to improve the antioxidant capacity of cells and consequently reduce arsenic-induced liver oxidative injury.关键词
葡萄籽原花青素提取物/砷/抗氧化活性/核因子E2相关因子Key words
grape seed proanthocyanidin extract/arsenic/antioxidant activity/nuclear factor erythroid-2 related factor 2 (Nrf2)分类
医药卫生引用本文复制引用
徐孟川,李述刚,丁玉松,牛强,冯刚玲,申卉,郭方明..葡萄籽原花青素提取物对砷诱导人肝细胞HL-7702损伤的保护作用及其机制[J].食品科学,2018,39(3):176-181,6.基金项目
国家自然科学基金地区科学基金项目(81560517 （）
81760584) （）
兵团应用基础研究项目(2015AG014) （2015AG014）
兵团科技领域重点攻关项目(2014BA039) （2014BA039）
石河子大学国际合作项目(GJHZ201602) （GJHZ201602）
2016年自治区研究生科研创新项目(XJGRI2016053) （XJGRI2016053）
