食品科学2018，Vol.39Issue(3)：222-228,7.DOI:10.7506/spkx1002-6630-201803034
白扁豆多糖对神经细胞缺氧性凋亡的保护机制
Protective Mechanism of Polysaccharides from Dolichos Bean Seeds(Dolichos lablab L.)on Hypoxia-Induced Neuronal Apoptosis
摘要
Abstract
Objective: The aim of this study was to explore the protective effect of dolichos bean seed polysaccharides (DBSP) against anoxia/reoxygenation (A/R) injury and its potential mechanism of action in neurons. Methods: Primary cultured cerebral cortical neurons were randomly divided into four groups including control group, A/R group, DBSP + A/R group and DBSP + LY294002 + A/R group. Cell survival rate and lactate dehydrogenase (LDH) release were determined. Apoptosis, mitochondrial membrane potential and Ca2+concentration were determined by flow cytometry analysis. The expression of T-Akt and p-Akt protein was evaluated by Western blot. Results: Compared with the control group, cell survival rate of the A/R group was decreased (P < 0.01) and LDH release, Ca2+concentration and apoptotic rate were significantly increased (P < 0.01). Compared with the A/R group, cell survival rate of the DBSP + A/R group was increased, and LDH release, Ca2+concentration and apoptotic rate were significantly decreased (P < 0.01), suggesting that DBSP could protect neurons against A/R injury. Mitochondrial membrane potential and the expression of p-Akt protein increased significantly in the DBSP +A/R group when compared with the A/R group (P < 0.01). The expression of p-Akt protein after treatment with LY294002 (a potent inhibitor of the PI3K-Akt signaling pathway) + DBSP + A/R was attenuated in neurons when compared with A/R + DBSP treatment (P < 0.05). Further experimental results showed that the treatment of cells with LY294002 completely blocked the expression of p-Akt protein, and significantly attenuated the protective effect of DBSP on neurons subjected to A/R. Conclusion: The protective mechanism of polysaccharides from dolichos bean seeds on neuronal cells is closely related to the PI3K-Akt signaling pathway.关键词
白扁豆多糖/神经细胞/缺氧/复氧/凋亡作用/PI3K-Akt信号通路Key words
dolichos bean seed polysaccharide/neuronal cells/anoxia/reoxygenation/apoptotic effect/PI3K-Akt signaling pathway分类
轻工纺织引用本文复制引用
张贤益,李文娟,钟亮,潘猛,汤小芳,姚于飞..白扁豆多糖对神经细胞缺氧性凋亡的保护机制[J].食品科学,2018,39(3):222-228,7.基金项目
南京军区面上项目(15MS086) （15MS086）
江西省自然科学基金重点项目(20171ACB21016) （20171ACB21016）
