食品科学2018，Vol.39Issue(4)：6-12,7.DOI:10.7506/spkx1002-6630-201804002
动植物源蛋白体外消化产物结构性质及ACE抑制活性
Structures and Angiotensin Converting Enzyme(ACE)Inhibitory Activity of in Vitro Digests of Animal and Plant Proteins
摘要
Abstract
This study aimed to reveal whether the difference in hypotensive activity between animal and plant proteins could be caused by the angiotensin conversion enzyme (ACE) inhibitory activity of peptides present in protein digests. The ACE inhibitory activity of peptides derived from in vitro digestion of plant and animal proteins was assessed.The in vitro digestion of plant proteins(rice,oat,soybean,and pea),red meat proteins(pork and beef),and white meat protein (chicken) was carried out by using a two-step process of hydrolysis with pepsin and trypsin. The hydrolysis degree, relative molecular weight distribution, amino acid composition and ACE inhibition rate of the obtained peptides were determined. The results showed that the inhibition percentage of ACE activity by protein digests increased with the increase in the degree of hydrolysis and consequently the decrease in the relative molecular weight. Among all protein digests, the ACE inhibition rate of plant protein digests was the highest (60.41%), while the lowest value (40.13%) was observed for red meat protein digests. The amino acid composition of protein digests showed that the levels of hydrophobic amino acids in both plant protein and white meat protein digests were significantly higher than in red meat protein digests. The higher the concentration of hydrophobic amino acids in protein digests was, the higher the ACE inhibitory activity was. It was suggested that the high content of hydrophobic amino acids may be one of the main causes of the ACE inhibitory activity of plant protein-derived peptides.关键词
植物蛋白/红肉蛋白/白肉蛋白/体外消化/血管紧张素转化酶Key words
plant protein/red meat protein/white meat protein/in vitro digestion/angiotensin converting enzyme(ACE)分类
化学化工引用本文复制引用
梁婷婷,佟立涛,蒲华寅,王丽丽,周闲容,鞠志远,周素梅,黄峻榕..动植物源蛋白体外消化产物结构性质及ACE抑制活性[J].食品科学,2018,39(4):6-12,7.基金项目
国家自然科学基金青年科学基金项目(31401505) （31401505）
陕西省科技统筹创新工程计划项目(2016KTCL02-23) （2016KTCL02-23）
中国农业科学院农产品加工研究所创新工程-谷物加工与品质调控团队项目 （）
