食品科学2018，Vol.39Issue(4)：138-143,6.DOI:10.7506/spkx1002-6630-201804021
超高效液相色谱-质谱法测定母乳中12种低聚糖
Determination of 12 Oligosaccharides in Human Milk by Ultra-Performance Liquid Chromatography-Mass Spectrometry
摘要
Abstract
A qualitative and quantitative method for the determination of 12 oligosaccharides in human milk was established. The conditions for gradient elution were set as follows: mobile phase A, 10 mmol/L ammonium formate solution;mobile phase B, acetonitrile; flow rate, 0.3 mL/min; and column temperature, 50 ℃. The gradient elution procedure was programmed as follows: 0–10 min, 95%–75% B; 10–15 min, 75% B; 15–20 min, 75%–65% B; 20–21 min, 65%–10% B;21–24 min, 10% B, 24–25 min, 10%–95% B; and 25–35 min, 95% B. The results showed that the standard curves of 12 oligosaccharides had good linearity within the concentration range investigated (R2> 0.98) and the recoveries were between 80.00% and 120.00%. The repeatability and instrumental precision (expressed as elative standard deviations) were less than 10%.关键词
低聚糖/母乳/超高效液相色谱-串联三重四极杆质谱Key words
oligosaccharides/human milk/ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-QQQ MS)分类
化学化工引用本文复制引用
陈新新,芦晶,刘鹭,逄晓阳,张书文,李诚,吕加平..超高效液相色谱-质谱法测定母乳中12种低聚糖[J].食品科学,2018,39(4):138-143,6.基金项目
国家自然科学基金青年科学基金项目(31401514) （31401514）
"十二五"国家科技支撑计划项目(2013BAD18B10) （2013BAD18B10）
现代农业产业技术体系北京市奶牛产业创新团队建设专项 （）
公益性行业(农业)科研专项(201303085) （农业）
