食品科学2018，Vol.39Issue(4)：194-198,5.DOI:10.7506/spkx1002-6630-201804029
植物源食品中α-胡萝卜素和β-胡萝卜素的测定及比较分析
Determination and Comparison of α-Carotene and β-Carotene of Plant-Derived Foods
摘要
Abstract
The study was conducted to develop an analytical method for simultaneous online determination of α-carotene and β-carotene in plant-derived foods by high performance liquid chromatography (HPLC). Furthermore, the method was validated with freeze-dried carrot powder. The results showed that the calibration curves for α-carotene and β-carotene were linear in the ranges of 0.8–20 and 0.4–8.2 μg/mL, respectively. The precision expressed as relative standard deviation (RSD) were 4.45% and 4.71%, respectively. The recoveries at three spiked levels were 96%–112% and 83.5%–103% with RSD values of 2.67%–5.83% and 4.90%–7.42%, respectively. These data indicated good repeatability and accuracy. Comparative analysis of various plant-derived foods by this method showed that β-carotene was more abundant than α-carotene in all samples except for pumpkin, which gave the opposite result.关键词
高效液相色谱法/胡萝卜素/α-胡萝卜素/β-胡萝卜素/植物源食品Key words
high-performance liquid chromatography/carotene/α-carotene/β-carotene/plant-derived foods分类
轻工纺织引用本文复制引用
崔亚娟,孔凡华,刘国杰,鲁绯,李东..植物源食品中α-胡萝卜素和β-胡萝卜素的测定及比较分析[J].食品科学,2018,39(4):194-198,5.基金项目
首都食品安全科技创优培育专项(Z141100002614013) （Z141100002614013）
